As of February 2022, there currently eight well-characterized published landing sites that have been created for RMCE insertion, of which : jsTi1453 I, jsSi1579 II, jsSi1669 IV, and jsTi1493 IV are very well characterized and highly efficient. An unpublished site on Chr V is also available. jsTi1453 and jsSi1579 are two component sites and require an unlinked source of FLP recombinase. We use bqSi711 IV as the source of FLP. jsSi1669 and jsTi1493 IV are single component landing sitse with a mex-5p FLP sl2 mNeonGreen cassette inserted into the landing site. jsSi1691 II is a single component version of jsSi1579, but is less efficient. jsTi1490 IV is closely linked to jsTi1493. We do not recommend using jsTi1492 II because the Flp sl2 mNeonGreen cassette of this insertion occasionally silences.
In addition, unpublished landing sites are available including one at jsSi1815 adjacent to the Chr V mosSCI oxTi365 site. If you don’t like any of these landing sites, it is possible to create new ones. Existing sites can be modified using RMCE. Novel landing sites can be created using a CRISPR SEC approach. Alternatively on can use miniMOS to isolate new insertions at random positions.