rRMCE vectors

General design:

Cloning compatibility:

–SpeI/SbfI/BstEII/SphI/KpnI/XmaI MCS

–TGG GTA SapI Golden Gate sites

–‘r’ plasmids swap SapI sites

This set of vectors includes vectors with different visible markers (nls-mNG, mNG, nls-GFP-C1, nls-Scarlet and nls-BFP), different drug selection options (HygR, NeoR, PuroR), different orientations of the drug selection marker and the polylinker. In addition, vectors without the loxP site are available which yield insertions that cannot have drug selection cassette excised even in the presence of Cre. The following are available upon request (or Addgene in Bold):

pHygG2(r)

[seq] | r [seq] | [map]

pHygG12

[seq]

pHygR2(r)

[seq] | r [seq] | [map]

pHygR12

[seq]

pHygCG2(r)

[seq] | r [seq] | [map]

pHygRR2(r)

[seq] | r [seq] | [map]

pHygG3(r)

[seq] | r [seq] | [map]

pHygCG3(r)

[seq] | r [seq] | [map]

pHygR3(r)

[seq] | r [seq] | [map]

pHygRR3(r)

[seq] | r [seq] | [map]

pHygG4

[seq]

pHygO2(r)

[seq] | r [seq] | [map]

pHygO3(r)

[seq] | r [seq] | [map]

pHygG6(r)

[seq] | r [seq] | [map]

pHygG7(r)

[seq] | r [seq] | [map]

pHygG8(r)

[seq] | r [seq] | [map]

pHygR8(r)

[seq] | r [seq] | [map]

pHygB1

[seq] [map]

pNeoG1(r)

[seq] | r [seq] |[map]

pNeoG2

[seq] | [map]

pNeoR2

[seq] |[map]

pPuroG1(r)

[seq] | r [seq] |[map]

pU119F1

[seq]

Contact Michael Nonet to request vectors.

Last updated 9-22-2023