RMCE landing sites:
Many of the strains on this page are available from the CGC. Those that are not can be directly requested from the Nonet lab. Click on allele to download genbank sequence file of insertion. These are described in Nonet (2020):
NM5161
jsTi1453 [mosL loxP rpl-28p FRT GFP-his-58 FRT3 mosR] I; bqSi711 [mex-5p::FLP::SL2::mNG + unc-119(+)] IV
NM5176
jsTi1490 [mosL loxP mex-5p FLP sl2 mNeonGreen rpl-28p FRT GFP-HIS-58 FRT3 mosR] IV
NM5178
jsTi1492 [mosL loxP mex-5p FLP sl2 mNeonGreen rpl-28p FRT GFP-HIS-58 FRT3 mosR] II
NM5179
jsTi1493 [mosL loxP mex-5p FLP sl2 mNeonGreen rpl-28p FRT GFP-HIS-58 FRT3 mosR] IV
NM5187
jsTi1453 I; him-8(e1489) IV
These are described in Nonet (2021b):
NM5322
jsTi1453 js1570 [∆mosL loxP rpl-28p FRT GFP-his-58 FRT3 mosR] I; bqSi711 [mex-5p::FLP::SL2::mNG + unc-119(+)] IV
- This landing site has the left miniMos arm deleted and may reduce background expression of bipartite system reporters.
NM5402
jsSi1579 [loxP rpl-28p FRT GFP-his-58 FRT3] II; bqSi711 [mex-5p::FLP::SL2::mNG + unc-119(+)] IV
- This dual component landing site in intergrated at a CRISPR site within 50 bp of ttTi5605. Integration into this site is more efficient in the presence of bqSi711 than integration into jsSi1691.
NM5500
jsSi1691 [loxP mex-5p FLP sl2 mNeonGreen rpl-28p FRT GFP-HIS-58 FRT3] II
- This single component landing site in intergrated at a CRISPR site within 50 bp of ttTi5605. However, it is less efficient (~1 per 10 animals) than integrating at the same position using NM5402 (Nonet, 2021b).
NM5471
jsSi1669 [loxP mex-5p FLP sl2 mNeonGreen rpl-28p FRT GFP-HIS-58 FRT3] IV
- This landing site is integrated at a CRISPR site within 50bp of the position of cxTi10882
The following site remains unpublished :
NM5738
jsSi1815 [loxP mex-5p FLP sl2 mNeonGreen rpl-28p FRT GFP-HIS-58 FRT3] V
- This landing site is integrated at a CRISPR site 279 bp from the position of oxTi365.
rRMCE landing sites:
Another set of landing sites compatible with RMCE are the sites created for rapid RMCE . Most of these have a much brighter pharyngeal cyOFP marker to track the landing site (which is lost during insertion). These landing sites do not provide additional chromosomal locations for insertions, but they should be easier to use if you do not have a fluorescent dissecting scope with a high power objective. These are unpublished but some are available at the CGC.
NM5548
jsSi1726 [loxP myo-2p FRT nlsCyOFP myo-2 3′ mex-5p FLP D5 glh-2 3′ FRT3] II
NM5549
jsSi1727 [mosL loxP myo-2p FRT nlsCyOFP myo-2 3′ mex-5p FLP D5 glh-2 3′ FRT3 mosR] I
NM5753
jsSi1837 [loxP mec-4Sp FRT nls-cyOFP mex-5p FLP D5 glh-2 3′ FRT3] IV
intermediate FRT10/FRT3 landing sites:
These landing sites are designed to modify FRT/FRT3 landing sites.
NM5861
jsSi1907 [loxP myo2p FRT10 mNG tbb-2 3′ mex-5p FLP D5 glh-2 3′ FRT3] I
NM5862
jsSi1908 [loxP myo2p FRT10 mNG tbb-2 3′ mex-5p FLP D5 glh-2 3′ FRT3] V
NM5893
jsSi1909 [loxP myo2p FRT10 mNG tbb-2 3′ mex-5p FLP D5 glh-2 3′ FRT3] IV
References
Nonet, 2020. Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics 215: 902-921.
Nonet M.L. 2021b. Additional Landing Sites for Recombination-Mediated Cassette Exchange in C. elegans. microPublications Biology, 10.17912/micropub.biology.000503.