RMCE integration vectors

Integration Plasmids

This plasmid is designed for conventional restriction enzyme cloning, as well as multi-fragment Gibson assembly and SapI Golden Gate cloning approaches. (Nonet, 2020).

pLF3FShC2 [map] [sequence]

This derivative of pLF3FShC contains a different 5′ SapI site allowing one to add distinct inserts using SapI Golden Gate cloning. (Unpublished, available upon request).

pLF3FShCr [map] [sequence]

This derivative of pLF3FShC contains the SapI sites in opposite orientation allowing one to create opposite orientation clones using the same SapI Golden Gate cloning entry vectors. (Unpublished, available upon request).

mec-4p GFP-C1 positive control plasmid

pLF3FShC mec-4p GFP-C1 tbb-2 3’UTR [map] [sequence] [addgene]

This positive control has been successfully integrated at 4 different landing sites. (Nonet, 2020).

Last edited 2-9-2022