Plasmids

NOTE: This page no longer lists every plasmid that has been provided to other labs by the Nonet lab. In particular, the Nonet lab has developed an extensive collection of plasmids for manipulating the C. elegans genome using recombinases. These vectors are described under Genome Manipulation, but are not included in this list. Please don’t hesitate to contact ML Nonet if you seek a plasmid and cannot find it on this website.

C. elegans | CRISPR/Cas9 | Mammalian Expression | Zebrafish

pSB103 (snb-1 rescuing genomic) [sequence] [map]
pSB150a (aka pSB102-Q68V) [sequence] [map]
pSB120.65 (SNB-1::GFP) [sequence] [map]
pSB121.65 mec-7p::SNB1::GFP) [sequence] [map]
pSB126.65 (punc-4::SNB-1::GFP [sequence] [map]
pSB6162 (SNB-1 expression vector) [sequence] [map]
pSAM1 (rpm-1 promoter ::GFP) [sequence] [map]
pSAM3 (GFP::RPM-1 ) [sequence] [map]
pSAM8 (RPM-1 genomic clone) [sequence] [map]
pSAM13 (pmec-3::RPM-1 clone) [sequence] [map]
pRIM3 (pmec-7::UNC-10-GFP) [sequence] [map]
pRIM5 (unc-10 promoter::GFP) [sequence] [map]
pRIM6 (unc-10 rescuing genomic) [sequence] [map]
pRIM8 (unc-10-GFP rescuing genomic clone) [sequence] [map]
pRIM10 (unc-10 del [Znfinger-PDZ] GFP) [sequence] [map]
pRIM27 (unc-10 del PDZ) [sequence] [map]
pRIM47 ( rab-3p GFP UNC-10-PDZ-C2A) [sequence] [map]
pGEX4T-QPDZC2A (GST -PDZ-C2A) [sequence][map]
pQRIMcDNA1 (full length unc-10 Rim cDNA) [sequence] [map]
pTX10 (pRSET UNC-64 syntaxin expression vector) [sequence] [map]
pTX4 ( UNC-64B syntaxin cDNA) [sequence] [map]
pGEX2T-TX (syntaxin-GST expression vector) [sequence] [map]
pTX21 (UNC-64 syntaxin rescuing genomic) [sequence] [map]
pTX34 (UNC-64A-GFP syntaxin fusion) [sequence] [map]
pTX35 (UNC-64A-GFP minigene fusion) [sequence] [map]
pTX37 (UNC-64B-GFP syntaxin fusion) [sequence] [map]
pTX38 (UNC-64C-GFP syntaxin fusion) [sequence] [map]
pTX39 (UNC-64C-GFP minigene fusion) [sequence] [map]
pTX41 (GFP:UNC-64 syntaxin fusion) [sequence] [map]
pST110 (pRSET SNT-1 cytoplasmic domain expression) [sequence] [map]
pST121 (Lorist 6 SNT-1 genomic clone) [sequence] [map]
pHO2dQSNP (RIC-4 expression) [sequence] [map]
pMK2 (small rab-3 genomic construct) [sequence] [map]
pMK4 (rab-3 rescuing genomic) [sequence] [map]
pAC2 acy-1(js127) lesion in genomic construct [sequence] [map]
pAC3 acy-1(L244S) lesion in genomic construct [sequence] [map]
pAC4 acy-1(js127) genomic region with BamHI site in front of ATG [sequence] [map]
pAC5 acy-1(js127) genomic coding region under snb-1 promoter [sequence] [map]
pAC6 acy-1(js127) genomic coding region under myo-3 promoter [sequence] [map]
pAC8 acy-1(js127) genomic coding region under glr-1 promoter [sequence] [map]
pAC9 acy-1(js127) genomic coding region under unc-25 promoter [sequence] [map]
prabGFPRim3′ (rab-3 promoter for neuronal expression) [sequence] [map]
pRAB100 (pan neuronal promoter /eGFP fusion construct) [sequence] [map]
GFPrab3Rim3′ (GFP::RAB-3 under the rab-3 promoter) [sequence] [map]
pmec-7GFP-RAB3-rim3′ (GFP::RAB-3 under the mec-7 promoter) [sequence] [map]
pmec7 Scarlet-RAB-3 (scarlet::RAB-3 under the mec-7 promoter) [sequence] [map]
pBHA-rab3 (rab3 two-hybrid vector) [sequence] [ map]
pBHA-rab3(Q81L) (rab3 two-hybrid vector) [sequence] [map]
pBHA-rab3(T36N) (rab3 two-hybrid vector) [sequence] [map]
pBK3.1 (slo-1 cDNA under snb-1 promoter) [sequence] [map]
pBK4 (slo-1cDNA:GFP fusion under myo-3 promoter [sequence] [map]
pBK4.1 (slo-1 cDNA under myo-3 promoter) [sequence] [map]
pmyo-3-egl-36(E142K) (egl-36(gf) cDNA under myo-3 promoter) [sequence] [map]
pmyo-3-egl-36(E142K G408E) (egl-36(lf) cDNA under the myo-3 promoter) [sequence] [map]
*note this plasmid is erroneously called pmyo-3-egl-36 (E142K G398E) in Zhao and Nonet 2000
pRSET/unc-13(1-267) (unc-13 zinc finger expression clone) [sequence] [map]
pPD95.75 SVOP (SVOP-1::GFP construct) [sequence] [map]
pSY1 (sng-1 promoter GFP construct) [sequence] [map]
pSY3 (SNG-1::GFP construct) [sequence] [map]
pSY12 (sng-1 pro moter::rat synaptogyrin::GFP) [sequence] [map]
pHSP-nsfGFP (CHO nsfGFP under hsp16 promoter) [sequence] [map]
pHSP-nsf(E329Q)GFP (CHO nsf(E329Q)GFP dn under hsp16 promoter) [sequence] [map]
hsp16-CeNSF::GFP (Ce NSFGFP under hsp16 promoter) [sequence] [map]
hsp16-CeNSF(E340Q)::GFP (Ce NSF(E340Q)GFP under hsp16 promoter) [sequence] [map]
pMU329 (myo-3promoter driving UNC-29) [sequence] [map]
pELKS 5 (ELKS-1 genomic clone) [sequence] [map]
pCAST06 (ELKS-1 genomic clone) [sequence] [map]
pELKS 9 (ELKS-1 del IWA genomic clone) [sequence] [map]
pELKS12 (ELKS-1 venus fusion) [sequence] [map]
ELKS cDNA clone (excision of yk329a12) [seqeuence] [map]
pmec-7 GFP-ELKS-1 Cbunc-119 [sequence][map]
NM990 vector [sequence]
pCFJ150 rab3GFPrab3 [sequence][map]
pBSKS(-) aex-4 genomic [sequence][map]
pET24b-APT-4 [sequence][map]
APT-4/pGEX-4T-1[sequence][map]
paex-2pCherry-Rim3′ [sequence][map]
pGADT7-unc-10 [sequence][map]
MCS-unc-104-GFP-Cbunc-119 [sequence][map]
NM2645 pCFK355K Pmec-7 mcherry gpd2/3 GFP-RAB-3 [sequence][map]
NM1269 pmec-7 mRFP unc-119 [sequence][map]
pCFJ151 pRAB-3 mcherry MCS unc-10 3’UTR [sequence][map]
pRF9 (rbf-1-GFP) [sequence][map]
sam-4pSAM-4TRFP unc-119 [sequence][map]
pCFJ178 sam-4pSAM-4-TRFP [sequence][map]
NM1860 mcherry-zxy-1 [sequence][map]
NM1874 zyx-1-GFP [sequence][map]
pSAPmec4p GAL4 QFAD [sequence][map]
TJ1353 [sequence][map]
phid-1 hid-1GFP [sequence][map]
prab-3 hid-1 GFP [sequence][map]
pges-1 hid-1GFP [sequence][map]
phid-1(G2S)hid-1 GFP [sequence][map]

RMCE

pLF3FShC (RMCE integration vector) [genbank] [map]
NM3746 DR274 CT-NT FLP LoxP FRT FRT3 landing [genbank][map]
NM3769 DR274 CT-NT loxP FRT FRT3 landing [genbank][map]

Cas9 Expression Clones

pHO4D-Cas9Nls (Cas9nls-his6 under control of T7 promoter for bacterial expression) [sequence] [map]
peft-3-cas-9 (a derivative of pDD162 that removes the U6 promoter sgRNA cassette) [sequence][map]

Worm U6 promoter sgRNA expression clones

DR274 U6 (C. elegans U6 promoter with BsaI sites for inserting targets ) [sequence][map]
DR274 U6 FE (C. elegans U6 promoterwith FE modified sgRNA and BsaI site for inserting targets) [sequence][map]

Mammalian Expression Clones

pSY26 (rat synaptogyrin::EGFP under CMV promoter) [sequence] [map]

Zebrafish Clones

pmT hb9s YFP [sequence][map]
pminiTol2 hb9syn YFP-rab3 [sequence][map]
UAS mcs YFP (14 UAS YFP) [sequence][map]
UAS-YFP-zfrab3 (14 UAS YFP-Zebrafish rab3 clone) [sequence] [map]
T2DT : a derivative of T2KSAG with a deletion of the tyrosinase sequences [sequence] [map]
T2DT UAS mcs YFP [sequence][map]
t2-DEST-GAL4VP16: a tol2 and Gateway vector for insertion of promoter in front of GAL4VP16. Derived from T2KSAG[sequence] [map]
pBH UAS YFPrab3 [sequence] [map]
pBH mcs [sequence] [map]
pBH mcs YFP [sequence] [map]
pBH YFP mcs [sequence] [map]
pBH UAS mcs YFP [sequence] [map]
pBH UAS YFP mcs [sequence] [map]
pBH Gtwy YFP [sequence] [map]
pBH mcs YFP Gtwy [sequence] [map]
pBH UAS Gtwy YFP [sequence] [map]
pBH UAS YFP Gtwy [sequence] [map]
pCH mcs [sequence] [map]
pCH mcs G4VP16 [sequence] [map]
pCH mcs G4m [sequence] [map]
pCH Gtwy G4VP16 [sequence] [map]
pCH Gtwy G4m [sequence] [map]
pGH mcs [sequence] [map]
pTAL7  wt Voytas (pTAL3) but with T7 promoter BGH 3’UTR [sequence] [map]
pTAL11 Voytas (?N?C)FokI (DKK) T7 promoter BGH 3’UTR [sequence] [map]
pTAL12 Voytas (?N?C)FokI (REL) T7 promoter BGH 3’UTR [sequence] [map]
pBH 4X UAS YFP mcs [sequence][map]
pBH 4xUAS TagRFP-mito [sequence][map]
pBH 5X UAS YFP mcs [sequence][map]
pmBH hb9s YFintP mcs inaa [sequence][map]
pCH mcs KalTA4GI [sequence][map]

last modified 8-3-2023 by M. L. Nonet