Figure 1 Overview of the available RMI vectors. The Multiple Cloning Site (MCS) region contains numerous common restrictions sites as well as SapI sites (arranged <SapI (TGG) SapI> (GTA) for Golden Gate cloning. The unique sites in the MCS differ slightly from vector to vector due to sites present in attB and attP. The attP vector contains a ‘full-sized’ attP site (Thorpe et al. 1998) and attB vectors have a 107 bp attB fragment which is significantly larger than the minimally defined sites (Groth et. al. 2000). The red and yellow lines in the att sites define the sites of phiC31-mediated recombination. The yellow triangles marked ‘L’ are loxP sites and the one marked ‘L2’ are lox2722 sites. The light green triangles marked ‘F’ are FRT sites and the dark green triangles marked ‘F3’ are FRT3 sites. The point of triangle denotes the orientation of these sites. The pAttB series vectors leave Amp and ori between the loxP site and the inertion after Cre excision, while the pAttB2 series vectors will leave only a very small spacer between loxP and the insertion. Generally, the series 2 vectors are the more logical to use unless you wish to introduce a large spacer between the insertion and the genomic region upstream of the insertion site. NeoC represents the C-terminal half of a split Neo gene. The features of the plasmids are not drawn to scale.
