Over view
Protein crosslinking is the process of chemically joining two amino acids residues through their side chain functional group reactivity by a covalent bond (crosslinking reagent). After protease treatment and LC-MS/MS interrogation, the cross linked (hybrid) peptides can be determined to high confidence.
Crosslinker selection, a big business
Commonly used crosslinkers in the lab and publication links: EDC, DTSSP, DSP, DSS, BS3
Isotopically coded crosslinkers (Creative Molecules)
Higher confidence and less false positives
Protein crosslinking software package
A case study of using isotope coded cross linker
Doublet of MS1: 4.0251X3=12.078 Da, mass shift of MS2 ion: 1213.75-1201.66=12.09 Da