SPINNING DISK CONFOCAL/COLIBRI 7
The Spinning Disk Confocal platform has extensive functionality for fast acquisition ranging from two-color live imaging to four-color tiling and multi-position capabilities. Some of the features of the system include the following:
·Spinning Disk Confocal for live imaging of up to 30 frames/second with 488 and 561nm laser lines.
·CO2 chamber with temperature control.
·Time Lapse image acquisition of live samples.
·Z-Sectioning of biospecimens for Orthogonal analysis and generation of Projections.
·Total Internal Reflective Fluorescence (TIRF) with 488nm laser.
·COLIBRI 7 with fast LED illumination at four excitation wavelengths.
·Motorized Stage in X, Y, and Z.
·Tiling and Multi-position capabilities for analysis of tissue sections, slides, and multi-well plates.
FEES
Zeiss Spinng Disk Confocal/Colibri 7
TRAINING/ORIENTATION $60/hr
CONFOCAL MICROSCOPE AND COMPUTER (Independent Use) $30/hr
LASERS
Laser Excitation Wavelength
488nm 50mW
561nm 50mW
MICROSCOPE
Zeiss Cell Observer Microscope (inverted)
FILTER SETS
DAPI, GFP, DsRed, and Cy5 modules
TEMPERATURE CONTROLLED CO2 CHAMBER
For live imaging
Plates that have been used successfully on this system for live imaging.
MatTek Corporation
35mm plates with glass coverslip bottom
P35G-0-14-C (0.85-0.13 mm thickness, 14mm diameter glass)
P35G-0-10-C (0.85-0.13 mm thickness, 10mm diameter glass)
www.GlassBottomDishes.com
Bioptechs, Inc.
35mm dishes with glass coverslip bottom
04200415C Delta T Dish 0.17mm clear
www.bioptechs.com
FINAL IMAGES
All images will be in zen format. These can be viewed with the ZEN software available on the Spinning Disk Observer computer. You may also view these on any PC using ZEN lite which can be downloaded free from the Zeiss website.
All users must go thru a training/orientation session prior to using any of the instrumentation.
Please contact Wandy Beatty for information or scheduling orientation/training sessions.
beatty@wustl.edu