The Zeiss LSM 880 Confocal Laser Scanning Microscope with Airyscan has a wealth of general functions for confocal image acquisition and analysis. In addition, the system is equipped with a heated stage for live analysis.
Some of the features of this system include the following:
·Colocalization of fluorescently-tagged components.
·Fluorescence Intensity Measurements of any given region of interest or profile.
·Z-Sectioning of biospecimens for Orthogonal analysis and generation of Projections.
·Time Lapse image acquisition of live samples.
·Fluorescence Resonance Energy Transfer (FRET) analysis of protein-protein interaction.
·Emission Fingerprinting for separation of overlapping emission spectra.
FEES
Zeiss LSM 880Confocal Laser Scanning Microscope
TRAINING/ORIENTATION $60/hr
CONFOCAL MICROSCOPE AND COMPUTER (Independent Use) $30/hr
COMPUTER ONLY (ZEN and VOLOCITY) for users of MMIF scopes NC
A second copy of the ZEN software is on a computer in an adjacent room.
LASERS
Laser Excitation Wavelength
Diode 405 nm
Argon 458, 488, 514 nm
HeNe1 543 nm
HeNe2 633 nm
MICROSCOPE
Zeiss Observer Microscope (inverted)
FILTER SETS
DAPI, GFP, DsRed, and Cy5 filter sets for fluorescence screening.
HEATED STAGE
For live imaging
Plates that have been used successfully on this system for live imaging.
MatTek Corporation
35mm plates with glass coverslip bottom
P35G-0-14-C (0.85-0.13 mm thickness, 14mm diameter glass)
P35G-0-10-C (0.85-0.13 mm thickness, 10mm diameter glass)
www.GlassBottomDishes.com
Bioptechs, Inc.
35mm dishes with glass coverslip bottom
04200415C Delta T Dish 0.17mm clear
www.bioptechs.com
FINAL IMAGES
All images will be in czi format. These can be viewed with the ZEN software available on the computer workstations. You may also view these on any PC using ZEN lite which can be downloaded free from the Zeiss website.
All users must go thru a training/orientation session prior to using any of the instrumentation.
Please contact Wandy Beatty for information or scheduling orientation/training sessions.
beatty@wustl.edu