2024
38. Sprunger M.L. and Jackrel M.E.^ (2024). The role of Matrin-3 in physiology and its dysregulation in disease. Biochem. Soc. Trans. link.
37. Lynch E.M., Pittman S., Daw J., Ikenaga C., Chen S., Dhavale D.D., Jackrel M.E., Ayala Y.M., Kotzbauer P., Ly C.V., Pestronk A., Lloyd T.E., and Weihl C.C. (2024). Seeding competent TDP-43 persists in human patient and mouse muscle. BioRxiv. link.
36. Chen S., Puri A., Bell B., Fritsche J., Palacios H.H., Balch M., Sprunger M.L., Howard M.K., Ryan J.J., Haines J.N., Patti G.J., Davis A.A., and Jackrel M.E.^ (2024). HTRA1 disaggregates α-synuclein amyloid fibrils and coverts them into non-toxic and seeding incompetent species. Nat. Commun. 15: 2436. link (^Corresponding author)
2023
35. Kim, Y., Li C., Gu C., Tycksen E., Puri A., Pietka T.A., Sivapackiam J., Fang Y., Kidd K., Park S., Johnson B.G., Kmoch S., Duffield J.S., Bleyer A.J., Jackrel M.E., Urano F., Sharma V., Lindahl M, and Chen Y.F. (2023). MANF stimulates autophagy and restores mitochondrial homeostasis to treat toxic proteinopathy. Nat. Commun. 14(1):6493. link.
34. Mack, K.L., Kim H., Barbieri E.M., Lin J., Braganza S., Jackrel M.E., DeNizio J.E., Yan X., Chuang E., Tariq A., Cupo R.R., Castellano L.M., Caldwell K.A., Caldwell G.A., and Shorter J. (2023). Tuning Hsp104 specificity to selectively detoxify α-synuclein. Mol Cell. 83(18):3314-3332.e9. pdf file link.
33. Shrimali, P.C., Chen S.*, Das A.*, Dreher R.D.*, Howard M.K.*, Ryan J.J., Buck J., Kim D., Sprunger M.L., Rudra J.S.^, and M.E. Jackrel^ (2023). Amyloidogenic propensity of self-assembling peptides and their adjuvant potential for use as DNA vaccines. Acta Biomaterialia. 169: 464-476. (*equal contributions, ^Co-corresponding author) pdf file link
32. Howard, M.K., Miller K.R., Sohn B.S., Ryan J.J., Xu A., and M.E. Jackrel^ (2023). Probing the drivers of Staphylococcus aureus biofilm protein amyloidogenesis and disrupting biofilms with engineered protein disaggregases. mBio. 10.1128/mbio.00587-23 (^Corresponding author) pdf file link
2022
31. Sprunger, M.L. and M.E. Jackrel^ (2022). Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching (FRAP). STAR Protoc. 3,101592. (^Corresponding author) pdf file link
30. Sprunger, M.L., K. Lee, B.S. Sohn, and M.E. Jackrel^ (2022). Molecular determinants and modifiers of Matrin-3 toxicity, condensate dynamics, and droplet morphology. iScience. 25(3):103900. (^Corresponding author) pdf file link
2021
29. Sprunger, M.L. and M.E. Jackrel^ (2021). Prion-like proteins in phase separation and their link to disease. Biomolecules. 11(7),1014. (^Corresponding author) pdf file link
28. Ryan, J.J., A. Bao, B. Bell, C. Ling, and M.E. Jackrel^ (2021). Drivers of Hsp104 potentiation revealed by scanning mutagenesis of the middle domain. Protein Science. 30(8):1667-1685. pdf file link (^Corresponding author)
2020
27. March, Z.M., K. Sweeney, H. Kim, X. Yan, L.M. Castellano, M.E. Jackrel, J. Lin, E. Chuang, E. Gomes, C. W. Willicott, K. Michalska, R. Jedrzejczak, A. Joachimiak, K.A. Caldwell, G.A. Caldwell, O. Shalem, and J. Shorter. (2020). Therapeutic genetic variation revealed in diverse Hsp104 homologs. eLife. 9:e57457. pdf file link
26. Howard, M.K., B.S. Sohn, J. von Borcke, A. Xu, and M.E. Jackrel^. (2020). Functional analysis of proposed substrate-binding residues of Hsp104. PLoS ONE. 15(3):e0230198. pdf file link (^Corresponding author)
2019
25. Tariq, A.*, J. Lin*, M.E. Jackrel*, C.D. Hesketh, P.J. Carman, K.L. Mack, R. Weitzman, C. Gambogi, O.A. Hernandez Murillo, E.A. Sweeny, E. Gurpinar, A.L. Yokom, S.N. Gates, K. Yee, S. Sudesh, J. Stillman, A.N. Rizo, D.R. Southworth, and J. Shorter. (2019). Mining disaggregase sequence space to safely counter TDP-43, FUS, and alpha-synuclein proteotoxicity. Cell Rep. 28(8):2080–2095. pdf file link (*Co-first author)
24. Sprunger, M.L. and M.E. Jackrel^. (2019). Quality control in the ER: Misfolded prohormones get a checkup. Mol. Cell. 75(3): 415-416. doi: https://doi.org/10.1016/j.molcel.2019.07.020. pdf file link (^Corresponding author)
23. Ryan, J.J., M.L. Sprunger, K. Holthaus, J. Shorter^ and M.E. Jackrel^. (2019). Engineered protein disaggregases mitigate toxicity of aberrant prion-like fusion proteins underlying sarcoma. J. Biol. Chem. doi: 10.1074/jbc.RA119.009494. pdf file link (^Co-corresponding author).
22. Durie, C.L, J. Lin, N.W. Scull, K.L. Mack, M.E. Jackrel, E.A. Sweeny, L.M. Castellano, J. Shorter, and A.L. Lucius (2019). Hsp104 and potentiated variants can operate as distinct nonprocessive translocases. Biophys J. link
21. Michalska, K., K. Zhang, Z.M. March, C. Hatzos-Skintges, G. Pintilie, L. Bigelow, L.M. Castellano, L.J. Miles, M.E. Jackrel, E. Chuang, R. Jedrzejczak, J. Shorter, W. Chiu, and A. Joachimiak. (2019). Structure of Calcarisporiella thermophila Hsp104 disaggregase that antagonizes diverse proteotoxic misfolding events. Structure. doi: 10.1016/j.str.2018.11.001. pdf file link
2018
20. Tariq, A., J. Lin, M.M. Noll, M.P. Torrente, K.L. Mack, O. Hernandez Murillo, M.E. Jackrel, and J. Shorter. (2018). Potentiating Hsp104 activity via phosphomimetic mutations in the middle domain. FEMS Yeast Res. 18(5)
doi: 10.1093/femsyr/foy042. pdf file link
19. Guo, L., H.J. Kim, H. Wang, J. Monaghan, F. Freyermuth, J.C. Sung, K. O’Donovan, C.M. Fare, Z. Diaz, N. Singh, Z.C. Zhang, M. Coughlin, E.A. Sweeny, M.E. DeSantis, M.E. Jackrel, C.B. Rodell, J.A. Burdick, O.D. King, A.D. Gitler, C. Lagier-Tourenne, U.B. Pandey, Y.M. Chook, J.P. Taylor, and J. Shorter. (2018). Nuclear-import receptors reverse aberrant phase transitions of RNA-binding proteins with prion-like domains. Cell. 173(3):677-692.
doi: 10.1016/j.cell.2018.03.002. pdf file link
2017
18. Gates, S.N., A.L. Yokom, J. Lin, M.E. Jackrel, A.N. Rizo, N.M. Kendsersky, C.E. Buell, E.A. Sweeny, K.L. Mack, E. Chuang, M.P. Torrente, M. Su, J. Shorter, and D.R. Southworth. (2017). Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104. Science. doi:10.1126/science.aan1052. pdf file link
17. Weaver, C.L., E.C. Duran, K.L. Mack, J. Lin, M.E. Jackrel, E.A. Sweeny, J. Shorter, and A.L. Lucius. (2017). Avidity for polypeptide binding by nucleotide-bound Hsp104 structures. Biochemistry. doi:10.1021/acs.biochem.7b00225. pdf file link
16. Jackrel, M.E.^, and J. Shorter.^ (2017). Protein-remodeling factors as potential therapeutics for neurodegenerative disease. Front. Neurosci. 11:99. pdf file (^Co-corresponding authors).
15. Yasuda, K., S.F. Clatterbuck-Soper, M.E. Jackrel, J. Shorter, and S. Mili. (2017). Cytoplasmic FUS inclusions disrupt Kinesin-1 leading to loss of detyrosinated microtubules and RNA mislocalization. J. Cell Biol. doi: 10.1083/jcb.201608022. pdf file link.
2016
14. Yokom, A.L., S.N. Gates, M.E. Jackrel, K.L. Mack, M. Su, J. Shorter, and D.R. Southworth. (2016). Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation. Nat. Struct. Mol. Biol. 23(9):830-7. pdf file link pdb emd
13. Torrente, M.P., E. Chuang, M.M. Noll, M.E. Jackrel, M.S. Go, and J. Shorter. (2016). Mechanistic insights into Hsp104 potentiation. J. Biol. Chem. 291(10):5101-5115. pdf file link
2015
12. Jackrel, M.E., K. Yee, A. Tariq, A.I. Chen, and J. Shorter. (2015). Disparate mutations confer therapeutic gain of Hsp104 function. ACS Chem. Biol. 10(12):2672-2679. pdf file link
11. Jackrel, M.E., and J. Shorter. (2015). Engineering enhanced protein disaggregases for neurodegenerative disease. Prion. 9(2):90-109. pdf file link
10. Sweeny, E.A., M.E. Jackrel, M.S. Go, M.A. Sochor, B.M. Razzo, M.E. DeSantis, K. Gupta, and J. Shorter. (2015). The Hsp104 N-terminal domain enables disaggregase plasticity and potentiation. Mol. Cell. 57(5):836-849. pdf file link
2014
9. Jackrel, M.E., A. Tariq, K. Yee, R. Weitzman, and J. Shorter. (2014). Isolating potentiated Hsp104 variants using yeast proteinopathy models. J. Vis. Exp. 93:e52089 doi:10.3791/52089. pdf file link
8. Jackrel, M.E., and J. Shorter. (2014). Potentiated Hsp104 variants suppress toxicity of diverse neurodegenerative disease-linked proteins. Dis. Model Mech. 7(10):1175-1184. pdf file link
7. Jackrel, M.E., and J. Shorter. (2014). Reversing deleterious protein aggregation with re-engineered protein disaggregases. Cell Cycle. 13(9):1379-1383. pdf file link
6. Jackrel, M.E., M.E. DeSantis, B.A. Martinez, L.M. Castellano, R.M. Stewart, K.A. Caldwell, G.A. Caldwell, and J. Shorter. (2014). Potentiated Hsp104 variants antagonize diverse proteotoxic misfolding events. Cell. 156(1-2):170–182. pdf file link
2012
5. DeSantis, M.E., E.H. Leung, E.A. Sweeny, M.E. Jackrel, M. Cushman-Nick, A. Neuhaus-Follini, S. Vashist, M.A. Sochor, M.N. Knight, and J. Shorter. (2012). Operational Plasticity Enables Hsp104 to Disaggregate Diverse Amyloid and Nonamyloid Clients. Cell. 151(4):778-793. pdf file link
2011
4. Jackrel, M.E., and J. Shorter. (2011). Shock and awe: unleashing the heat shock response to treat Huntington disease. J. Clin. Invest. 121(8):2972-2975. pdf file link
2010
3. Jackrel, M.E., A.L. Cortajarena, T.Y. Liu and L.Regan. (2010). Screening libraries to identify proteins with desired binding activities using a split-GFP reassembly assay. ACS Chem Biol. 5 (6): 553–562. pdf file link
2009
2. Jackrel, M.E., R. Valverde and L. Regan. (2009) Redesign of a protein – peptide interaction: characterization and applications. Protein Science. 18(4): 762-74. pdf file link
2008
1. Champion, E.A., B.H. Lane, M.E. Jackrel, L.Regan and S.J. Baserga. (2008). A direct interaction between the Utp6 half-a-tetratricopeptide repeat domain and a specific peptide in Utp21 is essential for efficient pre-rRNA processing. Mol Cell Biol. 28(21): 6547-56. pdf file link