Publications

2020

26. Howard, M.K., B.S. Sohn, J. von Borcke, A. Xu, and M.E. Jackrel^. (2020). Functional analysis of proposed substrate-binding residues of Hsp104. PLoS ONE. 15(3):e0230198. pdf file link (^Corresponding author)

2019

25. Tariq, A.*, J. Lin*, M.E. Jackrel*, C.D. Hesketh, P.J. Carman, K.L. Mack, R. Weitzman, C. Gambogi, O.A. Hernandez Murillo, E.A. Sweeny, E. Gurpinar, A.L. Yokom, S.N. Gates, K. Yee, S. Sudesh, J. Stillman, A.N. Rizo, D.R. Southworth, and J. Shorter. (2019). Mining disaggregase sequence space to safely counter TDP-43, FUS, and alpha-synuclein proteotoxicity. Cell Rep. 28(8):2080–2095. pdf file link (*Co-first author)

24. Sprunger, M.L. and M.E. Jackrel^. (2019). Quality control in the ER: Misfolded prohormones get a checkup. Mol. Cell. 75(3): 415-416. doi: https://doi.org/10.1016/j.molcel.2019.07.020. pdf file link (^Corresponding author)

23. Ryan, J.J., M.L. Sprunger, K. Holthaus, J. Shorter^ and M.E. Jackrel^. (2019). Engineered protein disaggregases mitigate toxicity of aberrant prion-like fusion proteins underlying sarcoma. J. Biol. Chem. doi: 10.1074/jbc.RA119.009494. pdf file (^Co-corresponding author).

22. Durie, C.L, J. Lin, N.W. Scull, K.L. Mack, M.E. Jackrel, E.A. Sweeny, L.M. Castellano, J. Shorter, and A.L. Lucius (2019). Hsp104 and potentiated variants can operate as distinct nonprocessive translocases. Biophys J. link

21. Michalska, K., K. Zhang, Z.M. March, C. Hatzos-Skintges, G. Pintilie, L. Bigelow, L.M. Castellano, L.J. Miles, M.E. Jackrel, E. Chuang, R. Jedrzejczak, J. Shorter, W. Chiu, and A. Joachimiak. (2019). Structure of Calcarisporiella thermophila Hsp104 disaggregase that antagonizes diverse proteotoxic misfolding events. Structure. doi: 10.1016/j.str.2018.11.001 pdf file link

2018

20. Tariq, A., J. Lin, M.M. Noll, M.P. Torrente, K.L. Mack, O. Hernandez Murillo, M.E. Jackrel, and J. Shorter. (2018). Potentiating Hsp104 activity via phosphomimetic mutations in the middle domain. FEMS Yeast Res. 18(5)
doi: 10.1093/femsyr/foy042. pdf file link

19. Guo, L., H.J. Kim, H. Wang, J. Monaghan, F. Freyermuth, J.C. Sung, K. O’Donovan, C.M. Fare, Z. Diaz, N. Singh, Z.C. Zhang, M. Coughlin, E.A. Sweeny, M.E. DeSantis, M.E. Jackrel, C.B. Rodell, J.A. Burdick, O.D. King, A.D. Gitler, C. Lagier-Tourenne, U.B. Pandey, Y.M. Chook, J.P. Taylor, and J. Shorter. (2018). Nuclear-import receptors reverse aberrant phase transitions of RNA-binding proteins with prion-like domains. Cell. 173(3):677-692.
doi: 10.1016/j.cell.2018.03.002. pdf file link

2017

18. Gates, S.N., A.L. Yokom, J. Lin, M.E. Jackrel, A.N. Rizo, N.M. Kendsersky, C.E. Buell, E.A. Sweeny, K.L. Mack, E. Chuang, M.P. Torrente, M. Su, J. Shorter, and D.R. Southworth. (2017). Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104. Science. doi:10.1126/science.aan1052. pdf file link

17. Weaver, C.L., E.C. Duran, K.L. Mack, J. Lin, M.E. Jackrel, E.A. Sweeny, J. Shorter, and A.L. Lucius. (2017). Avidity for polypeptide binding by nucleotide-bound Hsp104 structures. Biochemistry. doi:10.1021/acs.biochem.7b00225. pdf file link

16. Jackrel, M.E.^, and J. Shorter.^ (2017). Protein-remodeling factors as potential therapeutics for neurodegenerative disease. Front. Neurosci. 11:99. pdf file. (^Co-corresponding authors).

15. Yasuda, K., S.F. Clatterbuck-Soper, M.E. Jackrel, J. Shorter, and S. Mili. (2017). Cytoplasmic FUS inclusions disrupt Kinesin-­1 leading to loss of detyrosinated microtubules and RNA mislocalization. J. Cell Biol. doi: 10.1083/jcb.201608022. pdf file link.

2016

14. Yokom, A.L., S.N. Gates, M.E. Jackrel, K.L. Mack, M. Su, J. Shorter, and D.R. Southworth. (2016). Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation. Nat. Struct. Mol. Biol. 23(9):830-7. pdf file link pdb emd

13. Torrente, M.P., E. Chuang, M.M. Noll, M.E. Jackrel, M.S. Go, and J. Shorter. (2016). Mechanistic insights into Hsp104 potentiation. J. Biol. Chem. 291(10):5101-5115. pdf file link

2015

12. Jackrel, M.E., K. Yee, A. Tariq, A.I. Chen, and J. Shorter. (2015). Disparate mutations confer therapeutic gain of Hsp104 function. ACS Chem. Biol. 10(12):2672-2679. pdf file link

11. Jackrel, M.E., and J. Shorter. (2015). Engineering enhanced protein disaggregases for neurodegenerative disease. Prion. 9(2):90-109. pdf file link

10. Sweeny, E.A., M.E. Jackrel, M.S. Go, M.A. Sochor, B.M. Razzo, M.E. DeSantis, K. Gupta, and J. Shorter. (2015). The Hsp104 N-terminal domain enables disaggregase plasticity and potentiation. Mol. Cell. 57(5):836-849. pdf file link

2014

9. Jackrel, M.E., A. Tariq, K. Yee, R. Weitzman, and J. Shorter. (2014). Isolating potentiated Hsp104 variants using yeast proteinopathy models. J. Vis. Exp. 93:e52089 doi:10.3791/52089. pdf file link

8. Jackrel, M.E., and J. Shorter. (2014). Potentiated Hsp104 variants suppress toxicity of diverse neurodegenerative disease-linked proteins. Dis. Model Mech. 7(10):1175-1184. pdf file link

7. Jackrel, M.E., and J. Shorter. (2014). Reversing deleterious protein aggregation with re-engineered protein disaggregases. Cell Cycle. 13(9):1379-1383. pdf file link

6. Jackrel, M.E., M.E. DeSantis, B.A. Martinez, L.M. Castellano, R.M. Stewart, K.A. Caldwell, G.A. Caldwell, and J. Shorter. (2014). Potentiated Hsp104 variants antagonize diverse proteotoxic misfolding events. Cell. 156(1-2):170–182. pdf file link

2012

5. DeSantis, M.E., E.H. Leung, E.A. Sweeny, M.E. Jackrel, M. Cushman-Nick, A. Neuhaus-Follini, S. Vashist, M.A. Sochor, M.N. Knight, and J. Shorter. (2012). Operational Plasticity Enables Hsp104 to Disaggregate Diverse Amyloid and Nonamyloid Clients. Cell. 151(4):778-793. pdf file link

2011

4. Jackrel, M.E., and J. Shorter. (2011). Shock and awe: unleashing the heat shock response to treat Huntington disease. J. Clin. Invest. 121(8):2972-2975. pdf file link

2010

3. Jackrel, M.E., A.L. Cortajarena, T.Y. Liu and L.Regan. (2010). Screening libraries to identify proteins with desired binding activities using a split-GFP reassembly assay. ACS Chem Biol. 5 (6): 553–562. pdf file link

2009

2. Jackrel, M.E., R. Valverde and L. Regan. (2009) Redesign of a protein – peptide interaction: characterization and applications. Protein Science. 18(4): 762-74. pdf file link

2008

1. Champion, E.A., B.H. Lane, M.E. Jackrel, L.Regan and S.J. Baserga. (2008). A direct interaction between the Utp6 half-a-tetratricopeptide repeat domain and a specific peptide in Utp21 is essential for efficient pre-rRNA processing. Mol Cell Biol. 28(21): 6547-56. pdf file link