Department of Biochemistry & Molecular Biophysics
Washington University Biochemistry/Biophysics (WUBB) Community
BIOPHYSICAL EVENING

Single-Molecule Orientation-Localization Microscopy: New Challenges and Biophysical Insights

Abstract: Single-molecule orientation-localization microscopy builds upon photoswitching mechanisms used in localization microscopy (SMLM) to measure both the positions and orientations of fluorophores with nanoscale resolution. This 6D information enables biophysicists to visualize both the morphology and organization of biomolecular assemblies simultaneously. We have recently found that it is impossible for polarization-sensitive imaging to distinguish a single rotating (wobbling) molecule from a pair of molecules that spatially coincide, but measuring a fluorophore’s absorption and emission dipole moments simultaneously offers a solution to this challenge. I’ll also review our group’s recent use of fluorogenic probes to visualize the dynamic architectures of amyloid aggregates and biomolecular condensates.

Matthew Lew, PhD.
Associate Professor of Electrical & Systems Engineering
Washington University in Saint Louis

Date: Thurs, March 20th @ 4:00 p.m.
Location: Connor Auditorium, Farrell Learning & Teaching Center, 1st Floor
Reception to follow in 264 McDonnell Sciences

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Image credit: Oumeng Zhang