BIOL 5508
Offered Spring 2023
Course masters: Brian Clark, PhD and Philip Ruzycki, PhD
This course will introduce students to techniques used to study the visual system via discussions of foundational papers with experts who use these methods. Students will critically evaluate the literature to develop an understanding of the strengths and limitations of using these methods, which can then be used to plan experiments using these methods.
Students enrolled in the ITVS Pathway will also choose 2 hands-on demonstrations in instructor laboratories to participate in. These opportunities can be used to facilitate collaborations that can enhance students’ research.
Methods discussed include:
- Single-cell omics
- Viral engineering
- Genome engineering
- Epigenetics
- Electrophysiology
- In vivo imaging
- Automated behavior tracking/analysis
- Connectomics and image processing
Technique demonstrations available to ITVS students include:
- Create a single cell suspension and load 10X Genomics chip
- Create ATAC-seq library and primer on data analysis
- In vivo mouse electrophysiology recordings
- In vivo imaging of metabolic sensors in the mouse retina
- Mouse behavior tracking and primer on data analysis
- Image processing and analysis of 3D EM datasets