Solid-state nuclear magnetic resonance (NMR)
Confocal laser scanning microscopy (CLSM)
For reviews discussing the application of these technologies to study biofilms, refer to:
Reichhardt C. and L. Cegelski, Solid-state NMR for bacterial biofilms. Molecular Physics 2014, 112, 887-894.
Reichhardt C. and M.R. Parsek, Confocal laser scanning microscopy for analysis of Pseudomonas aeruginosa biofilm architecture and matrix localization. Frontiers Microbiology 2019, 10, 677.