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Washington University School of Medicine in St. Louis
Department of Genetics
Schedl Lab
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Germ Cell Book
Protocols
Protocols
EdU labeling and fixation
Ballistic Transformation
Ballistic Transformation
Supplement
Dissection, staining, in situ
Dissection, staining, in situ
Gonad Dissections
Gonad Fixation
DAPI Staining
Antibody Staining of Dissected Gonads
Activated MPK-1 Staining (MAPK-YT, Diphospho-MPK-1)
Germline in situ hybridization
Worm Powder
RNAi stuff
RNAi stuff
RNAi plates with lactose (instead of IPTG)
Feeding RNAi
dsRNA (double-stranded RNA) for injection
Genomic stuff
Genomic stuff
Worm genomic DNA prep
Single worm PCR
Mutagenesis stuff
Mutagenesis stuff
Mutagenesis with EMS
Mutagenesis with ENU
Transformation stuff
Transformation stuff
Yeast transformation
Electrocompetent cells
Heat shock transformation (DH10B homemade)
Protein stuff
Protein stuff
BSA conjugation with maleimide (Pierce)
Glycine elution (affinity purification)
IPTG induction
Kinase assay
Cytosolic extract & immunoprecipitation
Cytosolic extract using sonication (fast)
Lee Supplemental
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Protocols
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Protocols
EdU labeling and staining
Ballistic Transformation
Dissection, staining, in situ
RNAi stuff
Genomic stuff
Mutagenesis stuff
Transformation stuff
Protein stuff