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Washington University School of Medicine in St. Louis

Department of Genetics

Schedl Lab

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  • Germ Cell Book
  • Protocols
    • EdU labeling and fixation
    • Ballistic Transformation
      • Supplement
    • Dissection, staining, in situ
      • Gonad Dissections
      • Gonad Fixation
      • DAPI Staining
      • Antibody Staining of Dissected Gonads
      • Activated MPK-1 Staining (MAPK-YT, Diphospho-MPK-1)
      • Germline in situ hybridization
      • Worm Powder
    • RNAi stuff
      • RNAi plates with lactose (instead of IPTG)
      • Feeding RNAi
      • dsRNA (double-stranded RNA) for injection
    • Genomic stuff
      • Worm genomic DNA prep
      • Single worm PCR
    • Mutagenesis stuff
      • Mutagenesis with EMS
      • Mutagenesis with ENU
    • Transformation stuff
      • Yeast transformation
      • Electrocompetent cells
      • Heat shock transformation (DH10B homemade)
    • Protein stuff
      • BSA conjugation with maleimide (Pierce)
      • Glycine elution (affinity purification)
      • IPTG induction
      • Kinase assay
      • Cytosolic extract & immunoprecipitation
      • Cytosolic extract using sonication (fast)
  • Lee Supplemental

Germ Cell Book

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Front-matter_ToC_vol 757
Chapter 1
Chapter 2
Chapter 3
Chapter 4
Chapter 5
Chapter 6
Chapter 7		Chapter 8
Chapter 9
Chapter 10
Chapter 11
Chapter 12
Chapter 13
Chapter 14
Index vol 757

Contact the Lab

Tim Schedl, Ph.D
Department of Genetics
Campus Box #8232
Washington University School of Medicine
4566 Scott Ave.
St. Louis, MO 63110

PHONE: (314)362-6164 [lab]
FAX: (314)362-7855

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