Featured Publications

A bone-specific adipogenesis pathway in health and disease (Links to an external site)

A bone-specific adipogenesis pathway in health and disease
Bone marrow adipocytes accumulate with age and in diverse disease states. However, their origins and adaptations in these conditions remain unclear, impairing our understanding of their context-specific endocrine functions and relationship with surrounding tissues. In this study, by analyzing bone and adipose tissues in the lipodystrophic ‘fat-free’ mouse, we define a novel, secondary adipogenesis pathway […]

Neuroskeletal Effects of Chronic Bioelectric Nerve Stimulation in Health and Diabetes (Links to an external site)

Neuroskeletal Effects of Chronic Bioelectric Nerve Stimulation in Health and Diabetes
Background/Aims: Bioelectric nerve stimulation (eStim) is an emerging clinical paradigm that can promote nerve regeneration after trauma, including within the context of diabetes. However, its ability to prevent the onset of diabetic peripheral neuropathy (DPN) has not yet been evaluated. Beyond the nerve itself, DPN has emerged as a potential contributor to sarcopenia and bone disease; […]

Neural regulation of bone marrow adipose tissue (Links to an external site)

Neural regulation of bone marrow adipose tissue
Bone marrow adipose tissue (BMAT) is an important cellular component of the skeleton. Understanding how it is regulated by the nervous system is crucial to the study of bone and bone marrow related diseases. BMAT is innervated by sympathetic and sensory axons in bone and fluctuations in local nerve density and function may contribute to […]

A neuroskeletal atlas: spatial mapping and contextualization of axon subtypes innervating the long bones of C3H and B6 mice (Links to an external site)

A neuroskeletal atlas: spatial mapping and contextualization of axon subtypes innervating the long bones of C3H and B6 mice
Nerves in bone play well‐established roles in pain and vasoregulation and have been associated with progression of skeletal disorders including osteoporosis, fracture, arthritis and tumor metastasis. However, isolation of the region‐specific mechanisms underlying these relationships is limited by our lack of quantitative methods for neuroskeletal analysis and precise maps of skeletal innervation. To overcome these […]

Microneedles for ultrasensitive biomarker detection (Links to an external site)

Microneedles for ultrasensitive biomarker detection
The detection and quantification of protein biomarkers in interstitial fluid is hampered by challenges in its sampling and analysis. Here we report the use of a microneedle patch for fast in vivo sampling and on-needle quantification of target protein biomarkers in interstitial fluid. We used plasmonic fluor—an ultrabright fluorescent label—to improve the limit of detection […]

Immunostaining of Skeletal Tissues (Links to an external site)

Immunostaining of Skeletal Tissues
Immunostaining is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, then visualizing the bound antibody using a chromogen (immunohistochemistry or IHC) or fluorescence (immunofluorescence or IF). Unlike in situ hybridization, which identifies gene transcripts in cells, immunostaining identifies the products themselves and provides […]

Refreshable Nanobiosensor Based on Organosilica Encapsulation (Links to an external site)

Refreshable Nanobiosensor Based on Organosilica Encapsulation
Implantable and wearable biosensors that enable monitoring of biophysical and biochemical parameters over long durations are highly attractive for early and presymptomatic diagnosis of pathological conditions and timely clinical intervention. Poor stability of antibodies used as biorecognition elements and the lack of effective methods to refresh the biosensors upon demand without severely compromising the functionality […]

Bone marrow adipose tissue does not express UCP1 (Links to an external site)

Bone marrow adipose tissue does not express UCP1
Adipocytes within the skeleton are collectively termed bone marrow adipose tissue (BMAT). BMAT contributes to peripheral and local metabolism, however, its capacity for cell-autonomous expression of uncoupling protein 1 (UCP1), a biomarker of beige and brown adipogenesis, remains unclear. To overcome this, Ucp1-Cre was used to drive diphtheria toxin expression in cells expressing UCP1 (Ucp1Cre+/DTA+). Despite […]

Peripheral Neuropathy as a Component of Diabetic Skeletal Disease (Links to an external site)

Peripheral Neuropathy as a Component of Diabetic Skeletal Disease
The goal of this review is to explore clinical associations between peripheral neuropathy and diabetic bone disease and to discuss how nerve dysfunction may contribute to dysregulation of bone metabolism, reduced bone quality, and fracture risk. In addition, we address therapeutic and experimental considerations to guide patient care and future research evaluating the emerging relationship […]

Nerves in bone: evolving concepts in pain and anabolism. (Links to an external site)

Nerves in bone: evolving concepts in pain and anabolism.
This review provides a historical perspective of the field of skeletal neurobiology which highlights the diverse yet interconnected nature of nerves and skeletal health, particularly in the context of bone anabolism and pain. 

Exploiting Self-Capacitances for Wireless Power Transfer. (Links to an external site)

Exploiting Self-Capacitances for Wireless Power Transfer.
Conventional approaches for wireless power transfer rely on the mutual coupling (near-field or far-field) between the transmitter and receiver transducers. In this paper, we show that when the operational power-budget requirements are in the order of microwatts, a self-capacitance (SC)-based power delivery has significant advantages in terms of the power transfer-efficiency, receiver form-factor, and system […]

Evolution of the Marrow Adipose Tissue Microenvironment (Links to an external site)

Evolution of the Marrow Adipose Tissue Microenvironment
In this review, we discuss the initial evolution of MAT in vertebrate lineages while emphasizing comparisons to the development of peripheral adipose, hematopoietic, and skeletal tissues. We then apply these evolutionary clues to define putative functions of MAT. Lastly, we explore the regulation of MAT by two major components of its microenvironment, the extracellular matrix […]

2021

  • Idleburg C, Lorenz MR, DeLassus EN, Scheller EL, Veis DJ. (2021) Immunostaining of Skeletal Tissues. Methods Mol Biol. 2221:261-273.
  • Wang Z, Luan J, Seth A, Liu L, You M, Gupta P, Rathi P, Wang Y, Cao S, Jiang Q, Zhang X, Gupta R, Zhou Q, Morrissey JJ, Scheller EL, Rudra JS, Singamaneni S. (2021) Microneedle patch for the ultrasensitive quantification of protein biomarkers in interstitial fluid. Nature Biomed Engineering. 5:64-76.

2020

  • Tratwal J, Labella R, Bravenboer N, Kerckhofs G, Douni E,Scheller EL, Badr S, Karampinos DC, Beck-Cormier S, Palmisano B, Poloni A, Moreno-Aliaga MJ, Fretz J, Rodeheffer MS, Boroumand P, Rosen CJ, Horowitz MC, van der Eerden BCJ, Veldhuis-Vlug AG, Naveiras O. (2020) Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society. Feb 28;11:65. doi: 10.3389/fendo.2020.00065. 
  • Gupta R, Luan J, Chakrabartty S, Scheller EL, Morrissey J, Singamaneni S. (2020) Refreshable Nanobiosensor Based on Organosilica Encapsulation of Biorecognition Elements. ACS Appl Mater Interfaces. Feb 5;12(5):5420-5428. 
  • Suchacki KJ, Tavares AAS, Mattiucci D, Scheller EL, Papanastasiou G, Gray C, Sinton MC, Ramage LE, McDougald WA, Lovdel A, Sulston RJ, Thomas BJ, Nicholson BM, Drake AJ, Alcaide-Corral CJ, Said D, Poloni A, Cinti S, Macpherson GJ, Dweck MR, Andrews JPM, Williams MC, Wallace RJ, van Beek EJR, MacDougald OA, Morton NM, Stimson RH, Cawthorn WP. (2020) Bone marrow adipose tissue is a unique adipose subtype with distinct roles in glucose homeostasis. Nat Commun. 11(1):3097. PMCID: PMC7303125
  • Tratwal J, ID Bekri, C Boussema, N Kunz, R Sarkis, T Koliqi, S Rojas-Sutterlin, F Schyrr, DN Tavakol, V Campos, Scheller EL, R Sarro, C Bárcena, B Bisig, V Nardi, L de Leval, O Burri, O Naveiras. (2020) MarrowQuant in Aging and Aplasia: A Digital Pathology Workflow For Quantification of Bone Marrow Compartments in Histological Sections. Front Endocrinol. doi: 10.3389/fendo.2020.00480 PMCID: pending

2019

  • Wee NKY, Lorenz MR, Bekirov Y, Jacquin MF, Scheller EL. (2019) Shared Autonomic Pathways Connect Bone Marrow and Peripheral Adipose Tissues Across the Central Neuraxis. Front Endocrinol. Sep 27;10:668. doi: 10.3389/fendo.2019.00668.
  • Beeve AT, Brazill JB, Scheller EL. (2019) Peripheral neuropathy as a component of skeletal disease in diabetes. Curr Osteoporos Rep. Aug 7. doi: 10.1007/s11914-019-00528-8
  • Brazill JB, Beeve AT, Craft CS, Ivanusic JJ, Scheller EL. (2019) Nerves in bone: evolving concepts in pain and anabolism. J Bone Miner Res. Jun 27. doi: 10.1002/jbmr.3822. 
  • McCabe IC, Fedorko A, Meyers MG Jr, Leinninger G, Scheller E, McCabe LR. Novel leptin receptor signaling mutants identify location and sex-dependent modulation of bone density, adiposity, and growth. (2019) J Cell Biochem. 120(3):4398-4408. PMCID: PMC6336499
  • Zou W, Rohatgi N, Brestoff JR, Zhang Y, Scheller EL, Craft CS, Brodt MD, Migotsky N, Silva MJ, Harris CA, Teitelbaum SL. (2019) Congenital lipodystrophy induces severe osteosclerosis. PLoS Genet. Jun 24;15(6):e1008244. PMCID: 31233501
  • Robles H, Park S, Joens MS, Fitzpatrick JAJ, Craft CS, Scheller EL. (2019) Characterization of the bone marrow adipocyte niche with three-dimensional electron microscopy. Bone. 118:89-98. PMCID: PMC6063802
  • Scheller EL, Khandaker S, Learman BS, Cawthorn WP, Anderson LM, Pham HA, Robles H, Wang Z, Li Z, Parlee SD, Simon BR, Mori H, Bree AJ, Craft CS, MacDougald OA. (2019) Bone marrow adipocytes resist lipolysis and remodeling in response to β-adrenergic stimulation. Bone. 118:32-41. PMCID: PMC6062480
  • Alazzawi Y, Aono K, Scheller EL, Chakrabartty S. Exploiting Self-Capacitances for Wireless Power Transfer. (2019) IEEE Trans Biomed Circuits Syst. 13(2):425-434.
  • Silva MJ, Eekhoff JD, Patel T, Kenney-Hunt JP, Brodt MD, Steger-May K, Scheller EL, Cheverud JM. Effects of High-Fat Diet and Body Mass on Bone Morphology and Mechanical Properties in 1100 Advanced Intercross Mice. (2019) J Bone Miner Res. doi: 10.1002/jbmr.3648.

2018

  • Turecamo SE, Walji TA, Broekelmann TJ, Williams JW, Ivanov S, Wee NK, Procknow JD, McManus MR, Randolph GJ, Scheller EL, Mecham RP, Craft CS. (2018) Contribution of metabolic disease to bone fragility in MAGP1-deficient mice. Matrix Biol. 67:1-14.
  • Bauerle KT, Hutson I, Scheller EL, Harris CA. (2018) Glucocorticoid Receptor Signaling Is Not Required for In Vivo Adipogenesis. Endocrinology. 159(5):2050-2061
  • Li Z, Hardij J, Bagchi DP, Scheller EL, MacDougald OA. (2018) Development, regulation, metabolism and function of bone marrow adipose tissues. Bone. 110:134-140.
  • Craft CS, Li Z, MacDougald OA, Scheller EL. (2018) Molecular differences between subtypes of bone marrow adipocytes. Current Molecular Biology Reports. Mar;4(1):16-23.

2017

  • Craft CS, Wee NK, Scheller EL. (2017)  Adipocytes and Bone. Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. 8th Edition. 
  • Scheller EL, Hildebolt C, Civitelli R. (2017)  Oral Manifestations of Metabolic Bone Diseases. Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. 8th Edition. 
  • Cawthorn WP, Scheller EL. (2017) Editorial: Bone Marrow Adipose Tissue: Formation, Function, and Impact on Health and Disease. Front Endocrinol. May 29;8:112. 

2016

  • Cawthorn WP, Scheller EL, Parlee SD, Pham HA, Learman BS, Redshaw CM, Sulston RJ, Burr AA, Das AK, Simon BR, Mori H, Bree AJ, Schell B, Krishnan V, MacDougald OA. (2016) Expansion of Bone Marrow Adipose Tissue During Caloric Restriction Is Associated With Increased Circulating Glucocorticoids and Not With Hypoleptinemia. Endocrinology. 157(2):508-521. PMCID: PMC4733126
  • Scheller EL, Khoury B, Moller KL, Wee NK, Khandaker S, Kozloff KM, Abrishami SH, Zamarron BF, Singer K. (2016) Changes in skeletal integrity and marrow adiposity during high-fat diet and after weight-loss. Front Endocrinol. Jul 27;7:102. PMCID: PMC4961699
  • Walji TA, Turecamo SE, Sanchez AC, Anthony BA, Abou-Ezzi G, Scheller EL, Link DC, Mecham RP, Craft CS. (2016) Marrow adipose tissue expansion coincides with insulin resistance in MAGP1-deficient mice. Front Endocrinol. Jun 30;7:87. PMCID: PMC4928449
  • Sulston RJ, Learman BS, Zhang B, Scheller EL, Parlee SD, Simon BR, Mori H, Bree AJ, Wallace RJ, Krishnan V, MacDougald OA, Cawthorn WP. (2016) Increased circulating adiponectin in response to thiazolidinediones: investigating the role of bone marrow adipose tissue. Front Endocrinol. Sep 21;7:128. PMCID: PMC5030308
  • Scheller EL, Burr A, MacDougald OA, Cawthorn WP. (2016) Inside out: Bone marrow adipose tissue as a source of circulating adiponectin. Adipocyte. DOI: 10.1080/21623945.2016.1149269
  • Scheller EL, Cawthorn WP, Burr AA, Horowitz MC, MacDougald OA. (2016) Marrow adipose tissue: Trimming the Fat. Trends Endocrinol Metab. 27(6):392-403. PMCID: PMC4875855
  • Craft CS and Scheller EL. (2016) Evolution of the Marrow Adipose Tissue Microenvironment. Calcif Tissue Int. Doi: 10.1007/s00223-016-0168-9