Microneedles for ultrasensitive biomarker detection (Links to an external site)

The detection and quantification of protein biomarkers in interstitial fluid is hampered by challenges in its sampling and analysis. Here we report the use of a microneedle patch for fast in vivo sampling and on-needle quantification of target protein biomarkers in interstitial fluid. We used plasmonic fluor—an ultrabright fluorescent label—to improve the limit of detection […]

Immunostaining of Skeletal Tissues (Links to an external site)

Immunostaining is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, then visualizing the bound antibody using a chromogen (immunohistochemistry or IHC) or fluorescence (immunofluorescence or IF). Unlike in situ hybridization, which identifies gene transcripts in cells, immunostaining identifies the products themselves and provides […]

Postdoc Dr. Mohamed Hassan awarded RLM fellowship (Links to an external site)

Dr. Hassan was recently selected as the 2020 recipient of the Rita Levi-Montalcini Postdoctoral Fellowship in Regenerative Medicine by the Washington University Center of Regenerative Medicine. Dr. Hassan is a dentist-scientist with training in Orthodontics and will join the lab in the Summer of 2021 to begin his work. Congratulations, Mohamed!

Madelyn Lorenz unveils the Neuroskeletal Atlas at ASBMR (Links to an external site)

After 4 years of hard work, Madelyn Lorenz was selected to present her new Neuroskeletal Atlas of the Mouse Limb as a featured oral presentation at the American Society for Bone and Mineral Research (ASBMR) annual meeting. The Atlas is currently in preparation for publication and we anticipate having it available for preliminary use through […]

Xiao Zhang wins two research awards for work on marrow fat (Links to an external site)

Xiao Zhang won two of the prestigious awards for research at the recent BMA2020 meeting. After an independent committee review, his oral presentation “Fat in a fatless mouse: a novel origin of maladapted bone marrow adipocytes with age and disease” won 1st place in the best abstract category. In addition, his 2nd abstract “Neural contributions […]

Marrow adipose tissue has distinct roles in glucose homeostasis (Links to an external site)

Great to see this manuscript come together in the Cawthorn lab after many years of hard work, including his brand new BMAT imaging and analysis techniques and lots of lingering data generated by Drs. Cawthorn and Scheller when they worked together as postdocs in the MacDougald lab (Dr. Scheller made some of the blots in […]

Welcome to Mr. David Mingyu Shin

David Shin is currently a junior and was previously majoring in Economics and Accounting at Washington University in St. Louis prior to following the call to change his major to Biology. We welcome David to the lab and look forward to working with him to develop his scientific skills.

Refreshable Nanobiosensor Based on Organosilica Encapsulation (Links to an external site)

Implantable and wearable biosensors that enable monitoring of biophysical and biochemical parameters over long durations are highly attractive for early and presymptomatic diagnosis of pathological conditions and timely clinical intervention. Poor stability of antibodies used as biorecognition elements and the lack of effective methods to refresh the biosensors upon demand without severely compromising the functionality […]

Bone marrow adipose tissue does not express UCP1 (Links to an external site)

Adipocytes within the skeleton are collectively termed bone marrow adipose tissue (BMAT). BMAT contributes to peripheral and local metabolism, however, its capacity for cell-autonomous expression of uncoupling protein 1 (UCP1), a biomarker of beige and brown adipogenesis, remains unclear. To overcome this, Ucp1-Cre was used to drive diphtheria toxin expression in cells expressing UCP1 (Ucp1Cre+/DTA+). Despite […]

Welcome to Dr. Alexander Ahmadi and Xiao Zhang

A warm welcome to two new trainees.  Dr. Alexander Ahmadi joins us from the Saint Louis University (SLU) periodontics residency program.  Dr. Ahmadi will be working with us on the oral biosensor project.  Xiao Zhang is currently visiting as a rotation student from the BME program at Washington University. Xiao will investigate the properties and […]

Shared Autonomic Pathways Connect Bone and Adipose Tissue. (Links to an external site)

Bone marrow adipose tissue (BMAT) is increased in both obesity and anorexia. This is unique relative to white adipose tissue (WAT), which is generally more attuned to metabolic demand. It suggests that there may be regulatory pathways that are common to both BMAT and WAT and also those that are specific to BMAT alone. The […]

Postdoctoral fellow Dr. Natalie Wee presents at ASBMR (Links to an external site)

Dr. Wee was selected for a podium presentation for her work entitled “Shared autonomic pathways connect bone marrow and peripheral adipose tissues across the central neuraxis”. She presented during the session on ‘Bone-Lipid Connections’ at the recent 2019 ASBMR meeting. This work was also recently accepted for publication in Frontiers in Endocrinology. Congratulations Natalie!

Congratulations and farewell to Dr. Craft

Dr. Craft has worked with us since the founding of the lab in 2016 and her contributions have helped to shape the lab into what it is today. We are thankful for her science and her leadership during the past few years. We wish her all the best as she transitions into her new career!

Peripheral Neuropathy as a Component of Diabetic Skeletal Disease (Links to an external site)

The goal of this review is to explore clinical associations between peripheral neuropathy and diabetic bone disease and to discuss how nerve dysfunction may contribute to dysregulation of bone metabolism, reduced bone quality, and fracture risk. In addition, we address therapeutic and experimental considerations to guide patient care and future research evaluating the emerging relationship […]

Madelyn Lorenz and Alec Beeve present at ISAN 2019 (Links to an external site)

Members of the lab recently attended the 11th Congress of the International Society for Autonomic Neuroscience to share their science with other SPARC investigators and the autonomic neuroscience community. The lab presented two posters including “Nerves of the Bone: Foundational Neuroanatomical Mapping of Skeletal Nerves from Bone to Brain” (Lorenz) and “Acute and chronic responses […]

Alec Beeve appointed to the Skeletal Disorders Training Program (Links to an external site)

In addition to successfully passing his qualifying examination, Alec Beeve was recently appointed to a competitive graduate student fellowship position on the Skeletal Disorders Training Program (T32). Congratulations to Alec on his exam and his research fellowship to study the effects of acute and chronic bioelectric nerve stimulation on skeletal health.

Exploiting Self-Capacitances for Wireless Power Transfer. (Links to an external site)

Conventional approaches for wireless power transfer rely on the mutual coupling (near-field or far-field) between the transmitter and receiver transducers. In this paper, we show that when the operational power-budget requirements are in the order of microwatts, a self-capacitance (SC)-based power delivery has significant advantages in terms of the power transfer-efficiency, receiver form-factor, and system […]

Characterization of the bone marrow adipocyte niche with 3D-EM. (Links to an external site)

The bone marrow adipocyte (BMA) exists in a microenvironment containing unique populations of hematopoietic and skeletal cells. To study this microenvironment at the sub-cellular level, we performed a three-dimensional analysis of the ultrastructure of the BMA niche with focused ion beam scanning electron microscopy (FIB-SEM).

Bone marrow adipocytes resist lipolysis and β-adrenergic stimuli (Links to an external site)

Bone marrow adipose tissue (BMAT) is preserved or increased in states of caloric restriction. Similarly, we found that BMAT in the tail vertebrae, but not the red marrow in the tibia, resists loss of neutral lipid with acute, 48-hour fasting in rats. The mechanisms underlying this phenomenon and its seemingly distinct regulation from peripheral white […]

Jennifer Brazill begins T32 fellowship (Links to an external site)

Dr. Jennifer Brazill, a post-doctoral fellow with previous training in neurobiology, was appointed to the Skeletal Disorders Training Program T32 in the Division of Bone & Mineral Diseases at Washington University. For her fellowship, Dr. Brazill will study the physiologic integration of the neural and musculoskeletal systems in states of health and diabetes.

Evolution of the Marrow Adipose Tissue Microenvironment (Links to an external site)

In this review, we discuss the initial evolution of MAT in vertebrate lineages while emphasizing comparisons to the development of peripheral adipose, hematopoietic, and skeletal tissues. We then apply these evolutionary clues to define putative functions of MAT. Lastly, we explore the regulation of MAT by two major components of its microenvironment, the extracellular matrix […]