EXPERT PANEL

Timothy Schedl, PhD

Timothy Schedl, PhD

Professor of Genetics

Worm expert

Stephen Pak, PhD

Stephen Pak, PhD

Associate Professor of Pediatrics

Worm expert

Clifford Luke, PhD

Associate Professor of Pediatrics

CONSULTANTS

 

SERVICES

Instruments

Contact Stephen Pak (stephen.pak/at/wustl.edu) or Cliff Wolf (cjwolf/at/wustl.edu) for more information about instruments and resources

1. Microinjector

  • Used for generation of transgenic animals

2. Fluorescent stereo microscopes

  • 3x, for selection of transgenic animals and basic phentotype analysis

3. Leica SP8X scanning confocal microscope

  • With 405 and white light tunable lasers

4. ThermoFisher CellInsight CX-7 High-Content Imager

  • High throughput RNAi and drug screening
  • Automated phenotype quantification
  • Automated image acquisition in 96- and 384-well formats

5. BioTek Cytation 5 Imager

  • Similar to the CX-7

6. Biotek BIOSPA

  • Robotic microplate handler and live cell incubator

Other Resouces

Expression Vectors

Array of expression vectors of transgene expression in various tissues/cell types

Localization Vectors

Collection of vectors for subcellular protein localization studies (ER, lysosome, peroxisome, endosome, autophagic vesicles, etc)

Genome Editing

CRISPR/Cas9 genome editing

RNAi Library

C. elegans whole genome RNAi library (Ahringer)

Small Molecule Library

Compound libraries for drug screening and repurposing (Prestwick Library)

Current Collaborations

  1. A model of Alpha-1-antitrypsin deficiency (ATD) with Dr. David Perlmutter (Pediatrics)
  2. Drug screening using the C. elegans model of ATD with Dr. David Rudnick (Pediatrics)
  3. Modeling rare variants of ABC transporters with Drs. Sesh Cole and Jennifer Wambach (Pediatrics)
  4. Development of a C. elegans model of Wolfram Syndrome with Dr. Fumihiko Urano (Medicine – Endocrinology)
  5. A model of pancreatitis caused by accumulation of misfolded lipases with Drs. Mark Lowe and Xunjun Xiao (Pediatrics)
  6. A C. elegans model of Neimann-Pick Type C disease with Dr. Daniel Ory (Medicine – Cardiology)
  7. Undiagnosed Diseases Network – NIH-funded national network designed to functionally test the pathogenicity of human variants in model organisms.