Anabaena sp. PCC 33047

Developing a fast growing diazotrophic cyanobacterial platform for bioproduction

We are developing Anabaena sp. PCC 33047, a fast-growing, photosynthetic, diazotrophic cyanobacteria as a production platform for advanced biofuels and nitrogen-containing petrochemical replacement compounds. Anabaena 33047 displays a doubling time of 3.8 hours under photoautotrophic and N2-fixing conditions. It exhibits the highest recorded conversion rate of CO2 into biomass in an oxygenic photosynthetic organism (3.0 g L-1 day-1). This cyanobacterium is high light tolerant, fixes nitrogen at an unusually high rate, and grows well in sea water. We are using a systems biology approach for the development of this novel cyanobacterial strain into a platform organism for bioproduction.

Top - Light microscopy of Anabaena 33047. Bottom - chlorophyll fluorescence of Anabaena 33047. Heterocysts do not have PSII activity and did not fluoresce.

Growth of Anabaena 33047 under different light intensities. Intensities above 1500 µE m-2s-1 resulted in a 3.8-hour doubling time during logarithmic growth, but higher final culture densities are obtained at higher light intensities.

Professor Costas D. Maranas
The Pennsylvania State University
Maranas Lab Website

Professor Maciek R. Antoniewicz
University of Delaware
Antoniewicz Lab Website

This material is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomic Science Program under Award Number DE-SC0019386.