LOCUS       jsSi579_II_RMCE         5257 bp    DNA        linear       16-SEP-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..1383
                     /locus_tag="Mos insertion region"
                     /label="Mos insertion region"
                     /ApEinfo_label="Mos insertion region"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3839..3872)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1441..2042
                     /locus_tag="Prpl28"
                     /label="Prpl28"
                     /ApEinfo_label="Prpl28"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3433..3835
                     /locus_tag="UNC-54 3'UTR"
                     /label="UNC-54 3'UTR"
                     /ApEinfo_label="UNC-54 3'UTR"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3025..3390
                     /locus_tag="HIS-58"
                     /label="HIS-58"
                     /ApEinfo_label="HIS-58"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(2107..2277,2329..2482,2534..2697,2749..2973)
                     /locus_tag="GFP(65C)"
                     /label="GFP(65C)"
                     /ApEinfo_label="GFP(65C)"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1387..1437
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1398..1431
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2057..2090
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3875..5257
                     /locus_tag="Mos insertion region(1)"
                     /label="Mos insertion region(1)"
                     /ApEinfo_label="Mos insertion region"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2974..3024
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2278..2328
                     /locus_tag="intron(1)"
                     /label="intron(1)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#ff89d8"
                     /ApEinfo_revcolor="#ff89d8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2483..2533
                     /locus_tag="intron(2)"
                     /label="intron(2)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#ff89d8"
                     /ApEinfo_revcolor="#ff89d8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2698..2748
                     /locus_tag="intron(3)"
                     /label="intron(3)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#ff89d8"
                     /ApEinfo_revcolor="#ff89d8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGTCGGATTA TGGGCTCTTC TTTTATCCTC TACTTTCTTT CTAGAAAATG CAAATGTTGA
       61 GTCAGAAATT GCTGAATGGA GTACAACTGC AATGGGTTTA GCTTATGTTT CTGGAACAAT
      121 TACTGGTGGA ATTGTGATTG AAAAGTAAGG TTATATTTCA TTTCAATAAA TGTTACCACA
      181 GTATAACAAA AACAAGTTAC ATTGTTCAGA GTATGTCTGA ATGTATTATT ATTATTGTAT
      241 TTGTGCATCA GTACTGTAGT TGTACTGAAG AAGGGTTTAA AATAATCTCA ACGTTTTTAA
      301 GACGAGTTAG TGAGAATACG GTCGCAGTTA GGATGTTTGG ACATAGTTTT ATTAGATAAT
      361 CGTGAACTAA CTCACTATAA AAACCCGCTA ACTCATTGAA TAAGATAGTT TTAAACGTTC
      421 AAAATTTGAA GACAAAATTG TATGAATAAG TTTACAGAGT TGGTCGACGC AAGCTTCTCC
      481 TTCTCTTCAC ATTTCTCAAT AATTTAGCTC TAATTGCATT CGTATTCTTC GCAAAAATAC
      541 GTATTCTGAT TGACCCGATG AAATACGGAT GCCTAGTTGC ATTGATCATC TACGGTTATA
      601 CTTATGGGTG AGGTTTTTCA AATATATTTT GATTTAATCG AATAATGAAA TTTCAGAACT
      661 GGAGTTGGTC CAATTTCGTG GTTTATATCC TCTGAACTGG TACCTCAAAA ACATCGTAGT
      721 ATTGCTCAAT CTGTGGCATA TTCAATGAAT ACTGTAATGG TGGTTATCAG TACATTTACA
      781 GTATTACCTC TATATTCCGT TATTGGTAGT TATGCTTTCC TTATTCTATA TTCAATTCCT
      841 TCATTTATAA GTATGCTTAT ATTATTTCGC TACTTACCGG AAACCAAAGG ACGAGAGATT
      901 CATGATATTG TGAATGAACT TAAAAAAAAA TGAAAACCTT TGAAAACGTT CATACGGCCG
      961 AAATTTTACT TGATCGAGCC AATTGAACTT TTGTTAAGAC TTACAGAAAA GTGAGAGATT
     1021 TTGTTACAAA AAAGTGAGAA AAAAGCGAAA ATGAACAGGA AAAAGAAGAA AAATACACCC
     1081 GAAGTATCAT ATAAAAATTA AAAAGATTGT TTGACCTGGC GGAACTTTTT TGCAAAAACT
     1141 CTGAATATCC ATGGCACATG CTTCGTGCAA AACAGGATCC AAATGAATAT CAACAAGAGA
     1201 TTCTTGAAGA CGACGAGCCA CTTGCTCAGA GCATTGCTTA TCTCGAATGA GACCCTTGTT
     1261 AAAATCATTT TTCAAACATT ATATGACATT GTCGAAATGT CCTCCTGATT CCATGATGGT
     1321 AGCAAACTCA CTTCGTGTAA GTGCAAGTAA GATCAGTGTT TGTTTCGATA TCAGTCTGTT
     1381 TCGAAGgtaa gtttaaaATA ACTTCGTATA GCATACATTA TACGAAGTTA TtttcagGGT
     1441 GGGAGAAAAT GCGGAGAGAA GAACCCAGCA TCTTAATAAA ATTATATAAT TTTGAATAAA
     1501 ATCAAATTAA AAATAAGATT AATTGAAACA CAACAATAGA ATGCAGAAAA TTACTCATAA
     1561 GAAATAATTT ATTTTGAAAG AAGTTGTGTC GATTTACGAT AAAAACTACA CGCAAACGAG
     1621 GCTCCGCGTT TACACCGTTT GGCAATGGAG CGCGCTTGCT ATTAGATTTT AGGCCAGCTC
     1681 AACGAAAGTT TAATTTATTT TTCTTCTTTT TTTCCATTTT TTTGAGTAAA ATATCGAATT
     1741 TTTTGTATTA ATTCTGTAAA TTTTATAATT TGCAACTATT TTGGCGTATT CTGCTTGTTT
     1801 TTATCAGCGA ATTTTTATGC ATTTCAGTCA AAAGTTTGAG CAGGAAAAAT GACATTTAGG
     1861 ACTTTGTAAA ATTGTATTCT TAAATTTGCT TGAAAACCTA CGTGTACAAT TGATTTTATT
     1921 GAATTTTTCT TACGTTTCCC GTTGTATCTT ATTGTATTTA ACTTTCATCA TAAAATAATT
     1981 TATTTCCAGG TAAAATATCC GACGCTCTCG TGGATCCCCG GGATTGGCTC AGAAAAAAAG
     2041 GTACGAGCAG GCTTACGAAG TTCCTATTCT CTAGAAAGTA TAGGAACTTC GGTACCGGTA
     2101 GAAAAAATGA GTAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA
     2161 GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA
     2221 TACGGAAAAC TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGgta
     2281 agtttaaaca tatatatact aactaaccct gattatttaa attttcagCC AACACTTGTC
     2341 ACTACTTTCT GTTATGGTGT TCAATGCTTC TCGAGATACC CAGATCATAT GAAACGGCAT
     2401 GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA
     2461 GATGACGGGA ACTACAAGAC ACgtaagttt aaacagttcg gtactaacta accatacata
     2521 tttaaatttt cagGTGCTGA AGTCAAGTTT GAAGGTGATA CCCTTGTTAA TAGAATCGAG
     2581 TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG GACACAAATT GGAATACAAC
     2641 TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA AGAATGGAAT CAAAGTTgta
     2701 agtttaaaca tgattttact aactaactaa tctgatttaa attttcagAA CTTCAAAATT
     2761 AGACACAACA TTGAAGATGG AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA
     2821 ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT
     2881 TCGAAAGATC CCAACGAAAA GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT
     2941 GGGATTACAC ATGGCATGGA TGAACTATAC AAATGCCCGG GGGATCGGTG GAGCTCCACC
     3001 GGTGGCGGCC GCTCTAGAAC TAGTCCACCA AAGCCATCTG CCAAGGGAGC CAAGAAGGCC
     3061 GCCAAGACCG TCGTTGCCAA GCCAAAGGAC GGAAAGAAGA GACGTCATGC CCGCAAGGAA
     3121 TCGTACTCCG TCTACATCTA CCGTGTTCTC AAGCAAGTTC ACCCAGACAC CGGAGTCTCC
     3181 TCCAAGGCCA TGTCTATCAT GAACTCCTTC GTCAACGATG TATTCGAACG CATCGCTTCG
     3241 GAAGCTTCCC GTCTTGCTCA TTACAACAAA CGCTCAACGA TCTCATCCCG CGAAATTCAA
     3301 ACCGCTGTCC GTTTGATTCT CCCAGGAGAA CTTGCCAAGC ACGCCGTGTC TGAGGGAACC
     3361 AAGGCCGTCA CCAAGTACAC TTCCAGCAAG TAAACTAGTG GATCTGCAAC GCGTGGAGCT
     3421 AGCGCATCGC GAAGCTCCGC ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC
     3481 TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC
     3541 CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT
     3601 TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA
     3661 AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC
     3721 ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA
     3781 TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCGGA
     3841 AGTTCCTATA CtatttgaaG AATAGGAACT TCATGTAACG GTCTTCTGTA TAACTACAAA
     3901 AAAGAATAAA AAACCGTATC ACAACTGAGA AAAAAGACAA AACTTATTGA ACAGTGTTCC
     3961 TTTCAAAAAA TTATTTTGTA AAAAATCGAG TTTTTCTCCA AAATAACGCA CGCAATCAAA
     4021 AACAAGAAAA CTAACGTGTT CTCCTGGGCG TGGAACTCCT TATCAAGATT ACCATTATTG
     4081 TCTGTCAGAA TTGATATTTC AATTATTTCA TATCTAGACA AACCCGGGTG TCTTGCATTT
     4141 ATTAGTACTG TGAGTTTACA TACAAACGAG GCTTACTCAG ATAACATAGA GTCACCTCTA
     4201 ATTAGCAAAA ACCCATTTCT AATTAGCTAT TTCGACCAGT TCTAAAGTCA CCGTAATTAG
     4261 AAATGGTTTT TGTAGCTTTT CTAATTAGAA ATGACCCCGA GATAAGTGAA ATATGGCATT
     4321 TTCTGGGTTT TCTAGTAAGA AATGATCCCG CACGTAGTGA TTCCGCGTTA CAAGCCAAAT
     4381 TCAAATTAAA AAAGTATATT ACACAATTCA AGTTACATTA TTTTCAGGAA ACAATGGCGA
     4441 AGAGCAAAGC AGAGATTCAA AAGGAGGAAA GAGACAACAG ATATGAACGA AGACTTCTTG
     4501 AAAAGAGAAA GAGACATGAG GTGAGTAACC ATGATAATAA ATAATCAAGT AAGGGCTATA
     4561 GATTTATAAA TTCTGGAGTT AACAGTTTCG AAAAAAAGTA GTTTTCATAT GTGCGAAGGA
     4621 ATATCAAAAA TAGGGGAATA GCCTAAACGA GTGAGAAAAT TAAATGTTTG TTATTTTTTC
     4681 AAAAATGTCT GCAGGGCCGT TATCCTTTCA AATGCACTCT ATCAAACATA GATCATATCC
     4741 ATCATATCTT ATCCAGAATT TATATCATAT CCAAGATTGA GAGCGTTTCA AGGAAAATCT
     4801 CAAACAACCA TACAATTTTG TAGATCGACA ACAAGGCCTC CACTTCTTCA TCGATGGCCT
     4861 CTGCAGCACC TGCACCACCA AGTTTGTATT TTTCATATTA ATATAAAGTG AGCCACGCAT
     4921 AGTCGTTTTT AGGATTTTTG CCAGATCGAT GTCGCGCAAA CAGACTGTTT TTTTTTCTTT
     4981 GAAAGCCGTG CGTGACATGG GCCACGGATT GTAACGCCGC GCTGGATGGA TAAAAATTTA
     5041 TTCAGTTTTT TAGCCTTTAC AAAATATGGT TCGACTACTT TCTTTATTTT TACATTTTTA
     5101 TAGGTGCTCA TTGAGCTGAG ATATTTTGGG TATAATCGGA AAATCGCTGA TTTTGGTCAT
     5161 TTTTCTGCAC TAAAGATTAC ACAAAAAGTT CAGTATTTAG ATTCATTGTT GAAAATTGGG
     5221 GATGATCTAC AGATACATAT CGCCGTGTTC AAAAACA  
//
