Additional RMHE vectors

These RMHE compatible RMCE vectors have attB and attP sites on opposite sites of the MCS that the RMHE vectors we typically use, but they may have specialized uses.

Figure 1. Overview of unusual RMHE compatible RMCE vectors. The Master Cloning Site (MCS) contains numerous common restrictions sites as well as SapI sites for Golden Gate Cloning. Some vectors are available with the MCS SapI sites arranged <SapI (TGG) SapI> (GTA) and in the opposite orientation <SapI(TAC) SapI>(CCA). These are denoted with a terminal ‘r’ below. Some vectors contain ‘full-sized’ attB and attP sites (Thorpe et al. 1998) and other have minimally defined sites (Groth et. al. 2000).The unique sites in the MCS differ slightly from vector to vector due sites present in attB and attP. The red and yellow lines in the att sites define the sites of phiC31 mediated recombination. We do not recommend the use of these vectors since they are not compatible with the vast majority of transgenic attB and attP marked reporters and drivers we have created. See the RMHE vectors page for typical vectors

The original RMCE vector which is does not contain phiC31 att sites for recombination.

A derivative of pLF3FShC with a ‘full-sized’ attB on the 5′ end the insertion domain (the sequences between the loxP and FRT3 site that are inserted during RMCE). This is different from the configuration of the pRMHE vectors.

A derivative of pLF3FShC with a ‘full-sized’ attP site on the 3′ end of the insertion domain. This is different from the configuration of the pRMHE vectors.

Derivatives of pLF3FShC with a minimal attB site on the 3′ end of the insertion domain.

Derivatives of pLF3FShC with a minimal attP site on the 5′ end of the insertion domain.

References

  1. Groth, A. C., Olivares, E. C., Thyagarajan, B. & Calos, M. P. A phage integrase directs efficient site-specific integration in human cells. Proc Natl Acad Sci U S A 97, 5995-6000 (2000). PMID 10801973.
  2. Thorpe, H. M. & Smith, M. C. In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc Natl Acad Sci U S A 95, 5505-5510 (1998). PMID 9576912.

Last updated 12-1-2023