Dr. Holy’s research in imaging focuses on developing new optical methods for imaging neuronal activity in this lab. He has devised a new method, called objective-couple planar illumination microscopy, for imaging neuronal activity simultaneously in large neuronal populations. This approach uses a sheet of light to provide three-dimensional resolution without point-scanning. The principal advantage of this technique is that hundreds or thousands of neurons can be imaged at high speed and high signal-to-noise ratio. Current work on this technology includes optical and algorithmic methods for enhancing resolution deeper into tissue.