Nikon Eclipse 80i Fluorescent Microscope
The Nikon Eclipse 80i is a fixed-staged upright microscope for basic fluorescence imaging needs delivering high signal to noise ratios, which produces high quality fluorescence images. The microscope is configured to image in transmitted light, Differential Interference Contrast (DIC) and epifluorescence modes. This microscope is equipped with an epifluorescence attachment and 4 fluorescence filter cubes (DAPI, FITC, Texas Red, YFP) that are easily exchangeable to create different combinations. This microscope is not motorized.
Nikon Eclipse 80i upright microscope equipped with X-CITE 120XL mercury halide lamp channeled with a liquid light guide. Built-in – Watt white light source. Widefield 5.9 megapixel CMOS color microscope camera (new in 2016). Bright-field and Differential Interference Contrast capabilities.
| Objective | Magnification | NA | Working Distance |
|
Plan Fluor |
10X dry |
0.3 |
16 mm |
|
Plan Fluor |
40X dry |
0.75 |
0.66 mm |
|
Plan APO 60X oil |
60X oil immersion |
1.4 |
0.13 mm |
|
Plan APO |
100X oil immersion |
1.4 |
0.13 mm |
| Filter | Applications | Excitation Bandwidth | Emission Bandwidth |
|
UV-2E/C |
DAPI; Hoechst 33258/33342; |
340–380 nm |
435–485 nm |
|
B-2E/C |
FITC; Alexa488 |
465–495 nm |
515–555 nm
|
|
Y-2E/C |
Texas Red; Alexa 594 |
540-580 nm CWL: 560 nm |
600-660 nm CWL: 630 nm |
|
ET-YFP |
YFP |
480-520 nm CWL: 500 nm |
505-565 nm CWL: 535 nm |
Nikon DS-Fi3 5.9 megapixel CMOS color microscope camera (new in 2016)
2880X2048 pixel images at 15 fps, and 1440X1024 pixel images at 30 fps.
Nikon NIS Elements F4.60.00. Images are captured as .tiff