SRRF-Stream Imaging of Optogenetically Controlled Furrow Formation Shows Localized and Coordinated Endocytosis and Exocytosis Mediating Membrane Remodeling

2020

Castillo-Badillo JA, Bandi AC, Harlalka S, Gautam N.

ACS Synth Biol. 2020 Apr 17;9(4):902-919. doi: 10.1021/acssynbio.9b00521. Epub 2020 Mar 16.

PMID: 32155337 

Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration

2019

Meshik X, O’Neill PR, Gautam N

ACS Synth Biol. 2019 Mar 15;8(3):498-510. doi: 10.1021/acssynbio.8b00356. Epub 2019 Feb 22.

PMID: 30764607; PMCID: PMC708145

Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode.

2018

O’Neill PR, Castillo-Badillo JA, Meshik X, Kalyanaraman V, Melgarejo K, Gautam N
(2018) Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Developmental cell, 46(1):9-22.e4.

Optogenetic Control of Cell Migration

2018

Meshik X, O’Neill PR, Gautam N

Methods Mol Biol. 2018;1749:313-324. doi: 10.1007/978-1-4939-7701-7_22.

PMID: 29526006 

Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration.

O’Neill PR, Kalyanaraman V, Gautam N (2016) Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Molecular biology of the cell, ():E15-12-0832. [Epub ahead of print].

Optimizing optogenetic constructs for control over signaling and cell behaviours.

O’Neill PR, Gautam N (2015) Optimizing optogenetic constructs for control over signaling and cell behaviours. Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 14(9):1578-85.

Subcellular optogenetics – controlling signaling and single-cell behavior.

Karunarathne WK, O’Neill PR, Gautam N (2015) Subcellular optogenetics – controlling signaling and single-cell behavior. Journal of cell science, 128(1):15-25.

Descending control of itch transmission by the serotonergic system via 5-HT1A-facilitated GRP-GRPR signaling.

Zhao ZQ, Liu XY, Jeffry J, Karunarathne WK, Li JL, Munanairi A, Zhou XY, Li H, Sun YG, Wan L (2014) Descending control of itch transmission by the serotonergic system via 5-HT1A-facilitated GRP-GRPR signaling. Neuron, 84(4):821-34.

Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration.

O’Neill PR, Gautam N (2014) Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Molecular biology of the cell, 25(15):2305-14.

A G-protein subunit translocation embedded network motif underlies GPCR regulation of calcium oscillations.

Giri L, Patel AK, Karunarathne WK, Kalyanaraman V, Venkatesh KV, Gautam N (2014) A G-protein subunit translocation embedded network motif underlies GPCR regulation of calcium oscillations. Biophysical journal, 107(1):242-54.