Over the years we have generated many unique Drosophila melanogaster lines. Most of these contain P-element inserts with a visible reporter of Position Effect Variegation (PEV), most often an hsp70-driven white gene, designed to report on the local chromatin environment. Lines currently in our collection are listed below in association with the paper that describes the generation and characterization of these lines in greater detail (usually including determining the insertion site of the P-element reporter). (Most of these papers can be accessed through this website; see Research.) While some of these lines have been in continuous use, many others have not been used after the original publication. Therefore, we make no guarantees that the lines currently available are as originally reported. Through stock amplification and general maintenance over the years, the transposable elements are occasionally lost or altered; in some cases the phenotype is suppressed or enhanced, presumably due to drift or selection among the background chromosomes. In lines where drift (gradual change in phenotype) occurs over many generations, most often one see a suppression of PEV (greater expression of white at 25°C). The starting phenotype can generally be recovered by crossing the sibs exhibiting the strongest silencing (PEV) and/or backcrossing to a white minus stock. We recommend using inverse PCR to confirm the P element insertion site, and to check for additional critical genetic elements prior to using these lines in an investigation.
All lines are available now through May 2019, when the Elgin Drosophila lab will be closed. Please direct requests to Jo Wuller at wuller@wustl.edu, with a cc to Sarah Elgin (selgin@wustl.edu).
Initial lines exhibiting a PEV phenotype dependent on a heterochromatic environment: Lines labeled 39C or 118E are from Wallrath and Elgin, 1995, Genes Dev. 9:1263-77. PMID: 7758950. Lines labeled HS are from Cryderman et al, 1998, Chromosoma 107: 277-85 (PMID: 9880760). Further characterization of lines with a pericentric heterochromatin reporter (location C below) is in Cryderman et al 1998, and of lines with a telomere heterochromatin reporter (location T below) is in Cryderman et al 1999, EMBO J. 18:3724-35 (PMID 10393187). C, pericentric heterochromatin; T, telomeric or TAS heterochromatin, M, medial region of the fourth chromosome
| Name | Location | |
| 118E-X | X euchromatin | Homozygous Viable |
| 118E-3 | C 4 | Homozygous Viable |
| 118E-5 | T 4 | Homozygous Viable |
| 118E-9 | C 4 | Homozygous Viable |
| 118E-10 | C 4 | Homozygous Viable |
| 118E-12 | C 3R | Homozygous Viable |
| 118E-15 | T 4 | Homozygous Viable |
| 118E-16 | T 3R | Homozygous Viable |
| 118E-18 | T 4 | Homozygous Viable |
| 118E-19 | 102 B-C | Homozygous Viable |
| 118E-21 | subT 2R | Homozygous Viable |
| 118E-22 | T | Homozygous Viable |
| 118E-25 | C X | Homozygous Viable |
| 118E-25-1 | C X | Homozygous Viable |
| 118E-25-5 | 102D-E | Homozygous Viable |
| 118E-26 | T 3R | Homozygous Viable |
| 118E-28 | C X | Homozygous Viable |
| 118E-29 | C X | Homozygous Viable |
| 118E-32 | C X | Homozygous Viable |
| HS-1 | C 3L 79C-D | |
| HS-2 | C 3 | |
| HS-3 | C 3L 76E-77A | |
| HS-4 | T 4 | |
| HS-5 | C 2L | |
| HS-6 | T 4 | |
| 39C-X | X euchromatin | Homozygous Viable |
| 39C-2 | C 2R 42A-B | Homozygous Viable |
| 39C-3 | C 2L | Homozygous Viable |
| 39C-4 | C 3* | Homozygous Viable |
| 39C-5 | T 2L | Homozygous Viable |
| 39C-12 | 102D | Homozygous Viable |
| 39C-27 | T 2R | Homozygous Viable |
| 39C-31 | T 3R | Homozygous Viable |
| 39C-33 | M 4 | Homozygous Viable |
| 39C-34 | 102 E-F | Homozygous Viable |
| 39C-41 | C 4 | Homozygous Viable |
| 39C-42 | 102 B-C | Homozygous Viable |
| 39C-50 | T 2R | Homozygous Viable |
| 39C-51 | T 3R | Homozygous Viable |
| 39C-52 | 102 C | Homozygous Viable |
| 39C-55 | T 3R | Homozygous Viable |
| 39C-58 | T 2R | Homozygous Viable |
| 39C-61 | T 3R | Homozygous Viable |
| 39C-62 | T 3R | Homozygous Viable |
| 39C-63 | T 3R | Homozygous Viable |
| 39C-65 | T | Homozygous Viable |
| 39C-66 | Y | Homozygous Viable |
| 39C-68 | T 4 | Homozygous Viable |
| 39C-70 | C 4 | Homozygous Viable |
| 39C-71 | 4 | Homozygous Viable |
| 39C-72 | T 4 | Homozygous Viable |
| 39C-73 | T 3R | Homozygous Viable |
| 39C-74 | T | Homozygous Viable |
| 39C-18 | 102D-E | |
| 39C-28 | 102E-F | |
| 39C-33 | M 4 | |
| 39C-41 | C 4 | |
| 39C-76 | ? | |
| HS-7 | 102E |
*Not 2L as originally cytogenically mapped
Lines carrying an hsp70-white reporter inserted on the fourth chromosome but exhibiting a red eye phenotype are reported in Sun et al 2000, PNAS USA 97: 5340-5 (PMCID: PMC25830).
| Name |
| 1M 707-82 |
| 1M 707R/Ci[d] |
| 2M 59A-R/Ci[d] |
| 2M 663R/Ci[d] |
| 2M 371R/Ci[d] |
| 2M 390R/Ci[d] |
| 2M 530R-S/Ci[d] |
| 2M 626V/Ci[d] |
| 2M 823-S/Ci[d] |
| 2M 1021R/T(3.4) |
| 2M 802S/Ci[d] |
| 2M 913 Spa |
| 2M 010R 4rth Red #1 |
Screens designed to report on the local chromatin environment along the fourth chromosome produced many local insertions and deletions, as well as new insertions, as reported in Sun et al 2004 Mol Cell Biol 24: 8210-20 (DOI: 10.1128/MCB.24.18.8210-8220.2004) (series 4-6 below) and Riddle et al 2008 Genetics 178: 1177-91 (DOI: 10.1534/genetics.107.08.1828) (series 7-8 below). Screen #7 also recovered some additional variegating lines with insertions on other chromosomes.
| Name | |||
| 4M 33/Ci[d] | |||
| 4M 148V/Ci[d] | |||
| 4M 150V/Ci[d] | |||
| 4M 270V/Ci[d] | |||
| 4M 271/Ci[d] R/R | |||
| 4M 284V/Ci[d] | |||
| 4M 307V/Ci[d] | |||
| 4M 325/Ci[d] | |||
| 4M 344V/Ci[d] | |||
| 4M 348/Ci[d] | |||
| 4M 382V/Ci[d] | |||
| 4M 421V/Ci[d] | |||
| 4M 467R/Ci[d] | |||
| 4M 529V/Ci[d] | |||
| 4M 552V/Ci[d] | |||
| 4M 563V/Ci[d] | |||
| 4M 598V/Ci[d] | |||
| 4M 632V/Ci[d] | |||
| 4M 637V/Ci[d] | |||
| 4M 638/Ci[d] | |||
| 4M 660V/Ci[d] | |||
| 4M 667V/Ci[d] | |||
| 4M 759V/Ci[d] | |||
| 4M 779V/Ci[d] | |||
| 4M 838R/Ci[d] | |||
| 4M 871V/Ci[d] | |||
| 4M 919V/Ci[d] | |||
| 4M 935V/Ci[d] | |||
| 4M 979V/Ci[d] | |||
| 4M 993V/Ci[d] | |||
| 4M 1005V/Ci[d] | |||
| 4M 1030R/Ci[d] | |||
| 4M 1106V/Ci[d] | |||
| 4M 1107/Ci[d] | |||
| 4M 1207V/Ci[d] | |||
| 4M 1226V/Ci[d] | |||
| 4M 1277V/Ci[d] | |||
| 4M 1285R/Ci[d] | |||
| 4M 1323V/Ci[d] | |||
| 4M 1385V/Ci[d] | |||
| Name | |||
| 5M 140V/Ci[d] | |||
| 5M 153V/Ci[d] | |||
| 5M 165V/Ci[d] | |||
| 5M 187V/Ci[d] | |||
| 5M 188V/Ci[d] | |||
| 5M 197V/Ci[d] | |||
| 5M 199V/Ci[d] | |||
| 5M 201V/Ci[d] | |||
| 5M 234V/Ci[d] | |||
| 5M 237V/Ci[d] | |||
| 5M 241V/Ci[d] | |||
| 5M 244V/Ci[d] | |||
| 5M 263V/Ci[d] | |||
| 5M 272V/Ci[d] | |||
| 5M 275/Ci[d] | |||
| 5M 276V/Ci[d] | |||
| 5M 280V/Ci[d] | |||
| 5M 293V/Ci[d] | |||
| 5M 296V/Ci[d] | |||
| 5M 297V/Ci[d] | |||
| 5M 303V/Ci[d] | |||
| 5M 334V/Ci[d] | |||
| 5M 336V/Ci[d] | |||
| 5M 337V/Ci[d] | |||
| 5M 339V/Ci[d] | |||
| 5M 340/Ci[d] | |||
| 5M 341/Ci[d] | |||
| 5M 344V/Ci[d] | |||
| 5M 345V/(Ci[d]) P/P | |||
| 5M 349V/Ci[d] | |||
| 5M 350/Ci[d] | |||
| 5M 351V/Ci[d] | |||
| 5M 353V/Ci[d] | |||
| 5M 354/Ci[d] | |||
| 5M 358V/Ci[d] | |||
| 5M 359V/Ci[d] | |||
| 5M 361V/Ci[d] | |||
| 5M 363V/Ci[d] | |||
| 5M 298V on Y | |||
| 5M 309V/2475 on 2 | |||
| 5M 1V | |||
| 5M 42V | |||
| 5M 87V | |||
| 5M 91V | |||
| 5M 49 | |||
| 5M 8V | |||
| 5M 11V | |||
| 5M 63V | |||
| 5M 93V | |||
| 5M 97V | |||
| 5M 70V | |||
| 5M 15V | |||
| 5M 17V | |||
| 5M 72V | |||
| 5M 106V | |||
| 5M 107V | |||
| 5M 74V | |||
| 5M 19 | |||
| 5M 29V | |||
| 5M 84V | |||
| 5M 113V | |||
| 5M 119 | |||
| 5M 85V | |||
| 5M 34V | |||
| 5M 36V | |||
| 5M 133V | |||
| Name | |||
| 6-M 114 | |||
| 6-M 314 | |||
| 6-M 174 | |||
| 6-M 175 | |||
| 6-M 350 | |||
| 6-M 156v | |||
| 6-M 170 | |||
| 6-M 365 | |||
| 6-M 182 | |||
| 6-M 265 | |||
| 6-M 397 | |||
| 6-M 180v | |||
| 6-M 193v | |||
| 6-M 421 | |||
| 6-M 237 | |||
| 6-M 528v | |||
| 6-M 534 | |||
| 6-M 246 | |||
| 6-M 249 | |||
| 6-M 252 | |||
| 6-M 155v | |||
| 6-M 69 | |||
| 6-M 461v | |||
| 6-M 367 | |||
| 6-M 311v | |||
| 6-M 281v | |||
| 6-M 279 | |||
| Name | Notes | ||
| 7M 30/Ci[d] | |||
| 7M 53V/Ci[d] | |||
| 7M 201/Ci[d] | |||
| 7M 369/Ci[d] | |||
| 7M 484V/Ci[d] | |||
| 7M 547/Ci[d] | |||
| 7M 586/Ci[d] | |||
| 7M 972/Ci[d] | |||
| 7M 973/Ci[d] | |||
| 7M 1015/Ci[d] | |||
| 7M 1061V/Ci[d] | |||
| 7M 1067/Ci[d] | |||
| 7M 1079/(Ci[d]) P/P | |||
| 7M 1114/Ci[d] | |||
| 7M 1148V/Ci[d] | |||
| 7M 1244/(Ci[d]) P/P | |||
| 7M 1365V/Ci[d] | |||
| 7M 1399V/Ci[d] | |||
| 7M 116V | on 2 | ||
| 7M 415 | on Y | ||
| 7M 117V | on 2 | ||
| 7M 26 | on Y | ||
| 7M 27 | on Y | ||
| 7M 608V | on 3 | ||
| 7M 641V | on 3 | ||
| 7M 28 | on Y | ||
| 7M 29 | on Y | ||
| 7M 879V | on 3 | ||
| 7M 143V | on Y | ||
| 8M 100V | |||
| 8M 107 | |||
| 8M 112 on Y | |||
| 8M 114 on Y | |||
| 8M 115V | |||
| 8M 116V | |||
| 8M 117V | |||
| 8M 128V | |||
| 8M 130 | |||
| 8M 135 | |||
| 8M 136 | |||
| 8M 141 | |||
| 8M 152V | |||
| 8M 154V | |||
| 8M 167V | |||
| 8M 168V | |||
| 8M 175 | |||
| 8M 192 | |||
| 8M 199 | |||
| 8M 1V | |||
| 8M 207 | |||
| 8M 214V | |||
| 8M 227 | |||
| 8M 235 | |||
| 8M 262 | |||
| 8M 266 | |||
| 8M 285/(Ci[d]) P/P | |||
| 8M 28V | |||
| 8M 293 | |||
| 8M 309V | |||
| 8M 316/(Ci[d]) P/P | |||
| 8M 318/Ci[d] | |||
| 8M 435V-5 | |||
| 8M 56 | |||
| 8M 57V | |||
| 8M 66V-5 | |||
| 8M 68V | |||
| 8M 76 on Y | |||
| 8M 78V | |||
| 8M 83V | |||
| 8M 84V | |||
| 8M 90V-5 | |||
| 8M 91V-5 | |||
| 8M 94V | |||
| 8M 99V | |||
Screens designed to assess the impact of including a repetitious element (TE remnant 1360) along with hsp70-white in the P element reporter are reported in Haynes et al 2006 Curr Biol 16: 2222-7 (DOI: 10.1016/j.cub.2006.09.035) (T series below) and Huisinga et al 2016 Genetics 202: 565-82 (DOI: 10.1534/genetics.115.183228) (9m series below; 9m1 has one copy of 1360, while 9m4 has four copies of 1360). Haynes et al identifies a site (at the base of 2L) where silencing is dependent on the 1360. Huisinga et al shows that given inclusion of 1360, the P element inserts most often at the base of 2L and the TAS regions of 2R and 3R.
| Name | Notes |
| T190-177 | From Box XLV |
| T190-177 | Select PEV |
| ΔT190-177 | (-1360) |
| T190-212 | |
| ΔT1-90E/TM3 Sb | (-1360) |
| T1-36C | |
| T1-60F | (-1360) |
| T1-32E | |
| T190-168/Tm3 Sb | |
| ΔT1-60F | (-1360) |
Name
| 9m1-1 |
| 9m1-1012 |
| 9m1-105 |
| 9m1-137 |
| 9m1-170 |
| 9m1-201 |
| 9m1-23 |
| 9m1-246 |
| 9m1-270 |
| 9m1-273 |
| 9m1-287 |
| 9m1-287 #9 |
| 9m1-294 |
| 9m1-318 |
| 9m1-332 |
| 9m1-341 |
| 9m1-361 |
| 9m1-369 |
| 9m1-4 |
| 9m1-417 |
| 9m1-423 |
| 9m1-477 |
| 9m1-524 |
| 9m1-570 |
| 9m1-632 |
| 9m1-692 |
| 9M1-692 #13 |
| 9m1-7 |
| 9m1-752 |
| 9m1-770 |
| 9m1-786 |
| 9m1-827 |
| 9m1-851 |
| 9m1-891 |
| 9m1-912 |
| 9m1-929 |
| 9m1-940 |
| 9m1-971 |
| 9m1-981 |
| 9m1-99 |
| 9m1-996 |
| 9m4-10 |
| 9M4-104 |
| 9M4-11 |
| 9m4-12 |
| 9m4-128 |
| 9m4-13 |
| 9m4-157 |
| 9m4-163 |
| 9m4-166 |
| 9m4-17 |
| 9m4-18 |
| 9m4-185 |
| 9m4-19 |
| 9m4-2 |
| 9m4-20 |
| 9M4-212 |
| 9M4-212 |
| 9m4-212 |
| 9M4-214 |
| 9M4-214 |
| 9m4-214 |
| 9M4-217 |
| 9M4-217 |
| 9m4-217 |
| 9m4-220 |
| 9m4-222 |
| 9m4-226 |
| 9m4-23 |
| 9m4-24 |
| 9m4-241 |
| 9m4-247 |
| 9m4-25 |
| 9m4-26 |
| 9m4-27 |
| 9m4-278 |
| 9m4-282 |
| 9m4-289 |
| 9m4-292 |
| 9m4-293 |
| 9M4-31 |
| 9m4-313 |
| 9m4-33 |
| 9m4-340 |
| 9m4-340 |
| 9m4-340*-348 |
| 9m4-348*-340* |
| 9m4-349 |
| 9m4-35 |
| 9m4-353 |
| 9m4-36 |
| 9m4-364 |
| 9M4-366 |
| 9M4-366 |
| 9m4-366 |
| 9M4-37 |
| 9m4-379 |
| 9M4-39 |
| 9m4-397 |
| 9M4-4 |
| 9M4-40 |
| 9m4-41 |
| 9m4-45 |
| 9m4-47 |
| 9m4-472 |
| 9m4-486 |
| 9m4-487 |
| 9m4-490 |
| 9m4-506 |
| 9m4-51 |
| 9m4-525 |
| 9m4-54 |
| 9m4-558 |
| 9m4-56 |
| 9m4-560 |
| 9m4-564 |
| 9m4-574 |
| 9M4-58 |
| 9m4-580 |
| 9m4-584 |
| 9m4-59 |
| 9m4-595 |
| 9m4-598 |
| 9m4-6 |
| 9m4-602 |
| 9m4-61 |
| 9M4-62 |
| 9m4-626 |
| 9m4-629 |
| 9M4-63 |
| 9m4-632 |
| 9M4-64 |
| 9m4-640 |
| 9m4-644 |
| 9m4-647 |
| 9M4-65 |
| 9m4-656 |
| 9M4-73 |
| 9M4-74 |
| 9M4-76 |
| 9M4-77 |
| 9M4-79 |
| 9m4-8 |
| 9M4-80 |
| 9M4-81 |
| 9M4-82 |
| 9M4-84 |
| 9M4-85 |
| 9M4-87 |
| 9M4-88 |
| 9M4-89 |
| 9m4-9 |
| 9M4-92 |
| 9M4-95 |
The screen using a P element with a copy of 1360 adjacent to the hsp70-white reporter was repeated using a “landing pad” construct to recover a variegating reporter, dependent on 1360, that would allow analysis of critical elements for 1360-dependent silencing (Sentmanat & Elgin, 2012, PNAS USA 109: 14104-9 DOI: 10.1073/pnas.1207036109)
| Box # | Label | Notes | position | map, strand |
| Landing Pad Lines | 1171 | could be 1771 | X:3589639? | 3E, plus |
| Landing Pad Lines | 2201 | moderate PEV | 2L:21708198 | 39F1, plus |
| Landing Pad Lines | 1936 | moderate PEV | 2R:2301664 | 42A16, minus, piRNA cluster |
| Landing Pad Lines | 10 | weak PEV | 3L:3579448 | 64D14, minus |
| Landing Pad Lines | 1310 | strong PEV | 2L:21572961 | 39E, plus |
| Landing Pad Lines | 217 | moderate PEV | 2L:21540808 | 39E, plus |
| Landing Pad Lines | 1198 | weak PEV | 2L:20094149 | 38B6, plus |
| Landing Pad Lines #2 | 9-14 | solid red | 2L:3478435 | minus |
| Landing Pad Lines #2 | Line 6 | X chromosome | not mapped | |
| Landing Pad Lines #2 | Line 5 | weak PEV | 3R:27899517 | 100E, plus |
| Landing Pad Lines #2 | 9-19 | solid red | 4:328314 | minus |
| Landing Pad Lines #2 | 9-67 | solid red | 3R:5167563 | plus |
| Landing Pad Lines #2 | 9-100 | solid red | 3R:11636272 | plus |
| Landing Pad Lines #2 | 5-40 | 3L:16404893 | minus | |
| Landing Pad Lines #2 | 9-20 | peach | not mapped | |
| Landing Pad Lines #2 | 9-5 | strong PEV | 2L: 21572960 | minus |
| Landing Pad Lines #2 | 5-99 | solid red | 3R:15662765 | plus |
| Landing Pad Lines #2 | 4 | 3L:16404893 | plus | |
| Landing Pad Lines #2 | 5-33A | moderate PEV | 2R:7872227 | plus |
| Landing Pad Lines #2 | 5-4B | 3L16404893 | minus | |
| Landing Pad Lines #2 | 9-77 | X:633748 | plus | |
| Landing Pad Lines #2 | 5-98 | solid red | 3R:17459478 | plus |
As part of our study of the role of the piRNA system in silencing we created some lines carrying a piwi transgene mutated at V30A (Wang & Elgin, 2011, PNAS USA 108: 21164-9 DOI: 10.1073/pnas.1107892109).
| Name |
| w1118; piwi V30A #178.50 B1.2 |
| w1118; Piwi V30A #178.50 A3 |
| w1118; piwi V30A #178.50.C1 |
| w1118; piwi[WT] #RC12G2 |
Lines based on 1198 (Sentmanat & Elgin, 2012) that show repeat-induced silencing will become available after publication, later in 2019.