Over the years we have generated many unique Drosophila melanogaster lines.  Most of these contain P-element inserts with a visible reporter of Position Effect Variegation (PEV), most often an hsp70-driven white gene, designed to report on the local chromatin environment. Lines currently in our collection are listed below in association with the paper that describes the generation and characterization of these lines in greater detail (usually including determining the insertion site of the P-element reporter).  (Most of these papers can be accessed through this website; see Research.)  While some of these lines have been in continuous use, many others have not been used after the original publication.  Therefore, we make no guarantees that the lines currently available are as originally reported.  Through stock amplification and general maintenance over the years, the transposable elements are occasionally lost or altered; in some cases the phenotype is suppressed or enhanced, presumably due to drift or selection among the background chromosomes.  In lines where drift (gradual change in phenotype) occurs over many generations, most often one see a suppression of PEV (greater expression of white at 25°C).  The starting phenotype can generally be recovered by crossing the sibs exhibiting the strongest silencing (PEV) and/or backcrossing to a white minus stock. We recommend using inverse PCR to confirm the P element insertion site, and to check for additional critical genetic elements prior to using these lines in an investigation.

All lines are available now through May 2019, when the Elgin Drosophila lab will be closed.   Please direct requests to Jo Wuller at wuller@wustl.edu, with a cc to Sarah Elgin (selgin@wustl.edu).

Initial lines exhibiting a PEV phenotype dependent on a heterochromatic environment:  Lines labeled 39C or 118E are from Wallrath and Elgin, 1995, Genes Dev. 9:1263-77.  PMID: 7758950.  Lines labeled HS are from Cryderman et al, 1998, Chromosoma 107: 277-85 (PMID: 9880760).  Further characterization of lines with a pericentric heterochromatin reporter (location C below) is in Cryderman et al 1998, and of lines with a telomere heterochromatin reporter (location T below) is in Cryderman et al 1999, EMBO J. 18:3724-35 (PMID 10393187). C, pericentric heterochromatin; T, telomeric or TAS heterochromatin, M, medial region of the fourth chromosome

Name Location  
118E-X X euchromatin Homozygous Viable
118E-3 C 4 Homozygous Viable
118E-5 T 4 Homozygous Viable
118E-9 C 4 Homozygous Viable
118E-10 C 4 Homozygous Viable
118E-12 C 3R Homozygous Viable
118E-15 T 4 Homozygous Viable
118E-16 T 3R Homozygous Viable
118E-18 T 4 Homozygous Viable
118E-19 102 B-C Homozygous Viable
118E-21 subT 2R Homozygous Viable
118E-22 T Homozygous Viable
118E-25 C X Homozygous Viable
118E-25-1 C X Homozygous Viable
118E-25-5 102D-E Homozygous Viable
118E-26 T 3R Homozygous Viable
118E-28 C X Homozygous Viable
118E-29 C X Homozygous Viable
118E-32 C X Homozygous Viable
HS-1 C 3L 79C-D  
HS-2 C 3  
HS-3 C 3L 76E-77A  
HS-4 T 4  
HS-5 C 2L  
HS-6 T 4  
39C-X X euchromatin Homozygous Viable
39C-2 C 2R 42A-B Homozygous Viable
39C-3 C 2L Homozygous Viable
39C-4 C 3* Homozygous Viable
39C-5 T 2L Homozygous Viable
39C-12 102D Homozygous Viable
39C-27 T 2R Homozygous Viable
39C-31 T 3R Homozygous Viable
39C-33 M 4 Homozygous Viable
39C-34 102 E-F Homozygous Viable
39C-41 C 4 Homozygous Viable
39C-42 102 B-C Homozygous Viable
39C-50 T 2R Homozygous Viable
39C-51 T 3R Homozygous Viable
39C-52 102 C Homozygous Viable
39C-55 T 3R Homozygous Viable
39C-58 T 2R Homozygous Viable
39C-61 T 3R Homozygous Viable
39C-62 T 3R Homozygous Viable
39C-63 T 3R Homozygous Viable
39C-65 T Homozygous Viable
39C-66 Y Homozygous Viable
39C-68 T 4 Homozygous Viable
39C-70 C 4 Homozygous Viable
39C-71 4 Homozygous Viable
39C-72 T 4 Homozygous Viable
39C-73 T 3R Homozygous Viable
39C-74 T Homozygous Viable
39C-18 102D-E  
39C-28 102E-F  
39C-33 M 4  
39C-41 C 4  
39C-76 ?  
HS-7 102E  

*Not 2L as originally cytogenically mapped

Lines carrying an hsp70-white reporter inserted on the fourth chromosome but   exhibiting a red eye phenotype are reported in Sun et al 2000, PNAS USA 97: 5340-5 (PMCID: PMC25830).

Name
1M 707-82
1M 707R/Ci[d]
2M 59A-R/Ci[d]
2M 663R/Ci[d]
2M 371R/Ci[d]
2M 390R/Ci[d]
2M 530R-S/Ci[d]
2M 626V/Ci[d]
2M 823-S/Ci[d]
2M 1021R/T(3.4)
2M 802S/Ci[d]
2M 913 Spa
2M 010R 4rth Red #1

Screens designed to report on the local chromatin environment along the fourth chromosome produced many local insertions and deletions, as well as new insertions, as reported in Sun et al 2004 Mol Cell Biol 24: 8210-20 (DOI: 10.1128/MCB.24.18.8210-8220.2004) (series 4-6 below) and Riddle et al 2008 Genetics 178: 1177-91 (DOI: 10.1534/genetics.107.08.1828) (series 7-8 below).  Screen #7 also recovered some additional variegating lines with insertions on other chromosomes.

Name  
4M 33/Ci[d]  
4M 148V/Ci[d]  
4M 150V/Ci[d]  
4M 270V/Ci[d]  
4M 271/Ci[d] R/R  
4M 284V/Ci[d]  
4M 307V/Ci[d]  
4M 325/Ci[d]  
4M 344V/Ci[d]  
4M 348/Ci[d]  
4M 382V/Ci[d]  
4M 421V/Ci[d]  
4M 467R/Ci[d]  
4M 529V/Ci[d]  
4M 552V/Ci[d]  
4M 563V/Ci[d]  
4M 598V/Ci[d]  
4M 632V/Ci[d]  
4M 637V/Ci[d]  
4M 638/Ci[d]  
4M 660V/Ci[d]  
4M 667V/Ci[d]  
4M 759V/Ci[d]  
4M 779V/Ci[d]  
4M 838R/Ci[d]  
4M 871V/Ci[d]  
4M 919V/Ci[d]  
4M 935V/Ci[d]  
4M 979V/Ci[d]  
4M 993V/Ci[d]  
4M 1005V/Ci[d]  
4M 1030R/Ci[d]  
4M 1106V/Ci[d]  
4M 1107/Ci[d]  
4M 1207V/Ci[d]  
4M 1226V/Ci[d]  
4M 1277V/Ci[d]  
4M 1285R/Ci[d]  
4M 1323V/Ci[d]  
4M 1385V/Ci[d]  
Name  
5M 140V/Ci[d]  
5M 153V/Ci[d]  
5M 165V/Ci[d]  
5M 187V/Ci[d]  
5M 188V/Ci[d]  
5M 197V/Ci[d]  
5M 199V/Ci[d]  
5M 201V/Ci[d]  
5M 234V/Ci[d]  
5M 237V/Ci[d]  
5M 241V/Ci[d]  
5M 244V/Ci[d]  
5M 263V/Ci[d]  
5M 272V/Ci[d]  
5M 275/Ci[d]  
5M 276V/Ci[d]  
5M 280V/Ci[d]  
5M 293V/Ci[d]  
5M 296V/Ci[d]  
5M 297V/Ci[d]  
5M 303V/Ci[d]  
5M 334V/Ci[d]  
5M 336V/Ci[d]  
5M 337V/Ci[d]  
5M 339V/Ci[d]  
5M 340/Ci[d]  
5M 341/Ci[d]  
5M 344V/Ci[d]  
5M 345V/(Ci[d]) P/P  
5M 349V/Ci[d]  
5M 350/Ci[d]  
5M 351V/Ci[d]  
5M 353V/Ci[d]  
5M 354/Ci[d]  
5M 358V/Ci[d]  
5M 359V/Ci[d]  
5M 361V/Ci[d]  
5M 363V/Ci[d]  
5M 298V on Y  
5M 309V/2475 on 2  
5M 1V  
5M 42V  
5M 87V  
5M 91V  
5M 49  
5M 8V  
5M 11V  
5M 63V  
5M 93V  
5M 97V  
5M 70V  
5M 15V  
5M 17V  
5M 72V  
5M 106V  
5M 107V  
5M 74V  
5M 19  
5M 29V  
5M 84V  
5M 113V  
5M 119  
5M 85V  
5M 34V  
5M 36V  
5M 133V  
Name  
6-M 114  
6-M 314  
6-M 174  
6-M 175  
6-M 350  
6-M 156v  
6-M 170  
6-M 365  
6-M 182  
6-M 265  
6-M 397  
6-M 180v  
6-M 193v  
6-M 421  
6-M 237  
6-M 528v  
6-M 534  
6-M 246  
6-M 249  
6-M 252  
6-M 155v  
6-M 69  
6-M 461v  
6-M 367  
6-M 311v  
6-M 281v  
6-M 279  
Name Notes
7M 30/Ci[d]  
7M 53V/Ci[d]  
7M 201/Ci[d]  
7M 369/Ci[d]  
7M 484V/Ci[d]  
7M 547/Ci[d]  
7M 586/Ci[d]  
7M 972/Ci[d]  
7M 973/Ci[d]  
7M 1015/Ci[d]  
7M 1061V/Ci[d]  
7M 1067/Ci[d]  
7M 1079/(Ci[d]) P/P  
7M 1114/Ci[d]  
7M 1148V/Ci[d]  
7M 1244/(Ci[d]) P/P  
7M 1365V/Ci[d]  
7M 1399V/Ci[d]  
7M 116V on 2
7M 415 on Y
7M 117V on 2
7M 26 on Y
7M 27 on Y
7M 608V on 3
7M 641V on 3
7M 28 on Y
7M 29 on Y
7M 879V on 3
7M 143V on Y
8M 100V  
8M 107  
8M 112 on Y  
8M 114 on Y  
8M 115V  
8M 116V  
8M 117V  
8M 128V  
8M 130  
8M 135  
8M 136  
8M 141  
8M 152V  
8M 154V  
8M 167V  
8M 168V  
8M 175  
8M 192  
8M 199  
8M 1V  
8M 207  
8M 214V  
8M 227  
8M 235  
8M 262  
8M 266  
8M 285/(Ci[d]) P/P  
8M 28V  
8M 293  
8M 309V  
8M 316/(Ci[d]) P/P  
8M 318/Ci[d]  
8M 435V-5  
8M 56  
8M 57V  
8M 66V-5  
8M 68V  
8M 76 on Y  
8M 78V  
8M 83V  
8M 84V  
8M 90V-5  
8M 91V-5  
8M 94V  
8M 99V  
       

 Screens designed to assess the impact of including a repetitious element (TE remnant 1360) along with hsp70-white in the P element reporter are reported in Haynes et al 2006 Curr Biol 16: 2222-7 (DOI: 10.1016/j.cub.2006.09.035) (T series below) and Huisinga et al 2016 Genetics 202: 565-82 (DOI: 10.1534/genetics.115.183228) (9m series below; 9m1 has one copy of 1360, while 9m4 has four copies of 1360).  Haynes et al identifies a site (at the base of 2L) where silencing is dependent on the 1360.  Huisinga et al shows that given inclusion of 1360, the P element inserts most often at the base of 2L and the TAS regions of 2R and 3R.

Name Notes
T190-177 From Box XLV
T190-177 Select PEV
ΔT190-177 (-1360)
T190-212  
ΔT1-90E/TM3 Sb (-1360)
T1-36C  
T1-60F (-1360)
T1-32E  
T190-168/Tm3 Sb  
ΔT1-60F (-1360)

Name

9m1-1
9m1-1012
9m1-105
9m1-137
9m1-170
9m1-201
9m1-23
9m1-246
9m1-270
9m1-273
9m1-287
9m1-287 #9
9m1-294
9m1-318
9m1-332
9m1-341
9m1-361
9m1-369
9m1-4
9m1-417
9m1-423
9m1-477
9m1-524
9m1-570
9m1-632
9m1-692
9M1-692 #13
9m1-7
9m1-752
9m1-770
9m1-786
9m1-827
9m1-851
9m1-891
9m1-912
9m1-929
9m1-940
9m1-971
9m1-981
9m1-99
9m1-996
9m4-10
9M4-104
9M4-11
9m4-12
9m4-128
9m4-13
9m4-157
9m4-163
9m4-166
9m4-17
9m4-18
9m4-185
9m4-19
9m4-2
9m4-20
9M4-212
9M4-212
9m4-212
9M4-214
9M4-214
9m4-214
9M4-217
9M4-217
9m4-217
9m4-220
9m4-222
9m4-226
9m4-23
9m4-24
9m4-241
9m4-247
9m4-25
9m4-26
9m4-27
9m4-278
9m4-282
9m4-289
9m4-292
9m4-293
9M4-31
9m4-313
9m4-33
9m4-340
9m4-340
9m4-340*-348
9m4-348*-340*
9m4-349
9m4-35
9m4-353
9m4-36
9m4-364
9M4-366
9M4-366
9m4-366
9M4-37
9m4-379
9M4-39
9m4-397
9M4-4
9M4-40
9m4-41
9m4-45
9m4-47
9m4-472
9m4-486
9m4-487
9m4-490
9m4-506
9m4-51
9m4-525
9m4-54
9m4-558
9m4-56
9m4-560
9m4-564
9m4-574
9M4-58
9m4-580
9m4-584
9m4-59
9m4-595
9m4-598
9m4-6
9m4-602
9m4-61
9M4-62
9m4-626
9m4-629
9M4-63
9m4-632
9M4-64
9m4-640
9m4-644
9m4-647
9M4-65
9m4-656
9M4-73
9M4-74
9M4-76
9M4-77
9M4-79
9m4-8
9M4-80
9M4-81
9M4-82
9M4-84
9M4-85
9M4-87
9M4-88
9M4-89
9m4-9
9M4-92
9M4-95

 The screen using a P element with a copy of 1360 adjacent to the hsp70-white reporter was repeated using a “landing pad” construct to recover a variegating reporter, dependent on 1360, that would allow analysis of critical elements for 1360-dependent silencing (Sentmanat & Elgin, 2012, PNAS USA 109: 14104-9  DOI: 10.1073/pnas.1207036109)

Box # Label Notes position map, strand
Landing Pad Lines 1171 could be 1771 X:3589639? 3E, plus
Landing Pad Lines 2201 moderate PEV 2L:21708198 39F1, plus
Landing Pad Lines 1936 moderate PEV 2R:2301664 42A16, minus, piRNA cluster
Landing Pad Lines 10 weak PEV 3L:3579448 64D14, minus
Landing Pad Lines 1310 strong PEV 2L:21572961 39E, plus
Landing Pad Lines 217 moderate PEV 2L:21540808 39E, plus
Landing Pad Lines 1198 weak PEV 2L:20094149 38B6, plus
Landing Pad Lines #2 9-14 solid red 2L:3478435 minus
Landing Pad Lines #2 Line 6   X chromosome not mapped
Landing Pad Lines #2 Line 5 weak PEV 3R:27899517 100E, plus
Landing Pad Lines #2 9-19 solid red 4:328314 minus
Landing Pad Lines #2 9-67 solid red 3R:5167563 plus
Landing Pad Lines #2 9-100 solid red 3R:11636272 plus
Landing Pad Lines #2 5-40   3L:16404893 minus
Landing Pad Lines #2 9-20 peach not mapped  
Landing Pad Lines #2 9-5 strong PEV 2L: 21572960 minus
Landing Pad Lines #2 5-99 solid red 3R:15662765 plus
Landing Pad Lines #2 4   3L:16404893 plus
Landing Pad Lines #2 5-33A moderate PEV 2R:7872227 plus
Landing Pad Lines #2 5-4B   3L16404893 minus
Landing Pad Lines #2 9-77   X:633748 plus
Landing Pad Lines #2 5-98 solid red 3R:17459478 plus

As part of our study of the role of the piRNA system in silencing we created some lines carrying a piwi transgene mutated at V30A (Wang & Elgin, 2011, PNAS USA 108: 21164-9  DOI: 10.1073/pnas.1107892109).

Name
w1118; piwi V30A #178.50 B1.2
w1118; Piwi V30A #178.50 A3
w1118; piwi V30A #178.50.C1
w1118; piwi[WT] #RC12G2

Lines based on 1198 (Sentmanat & Elgin, 2012) that show repeat-induced silencing will become available after publication, later in 2019.