Welcome to the DNA Sequencing Innovation Lab & Spike-In Cooperative at WashU Medicine website.
We are part of The Edison Family Center for Genome Sciences & Systems Biology (CGS&SB) and provide services to labs at WashU Medicine, the Danforth campus, and off-site labs.
The DSIL is a small to medium-throughput sequencing facility that mainly assists WashU researchers. We specialize in sequencing innovations, custom applications, and the Spike-In program. We collaborate with the High-Throughput Computing Facility (HTCF) and GTAC@MGI to deliver sequencing data to our users.
Overview of Requirements and Services
Step 1:
Make a standard Illumina library with a minimum of 20 uL at 10 nM.
Step 2:
Submit the library to the DSIL. Samples received after 1 pm will be processed the following business day.
Follow-up:
- You will receive demultiplexed fastq files of your sample(s).
- Quick median turnaround: 3 days for spike-in runs, and less than one week for full runs.
Find out more about our Spike-In Cooperative program.
Our DSIL Experts
Jess Hoisington-López
Research Lab Manager
Jessica Hoisington-López has been with the DSIL since 2008, after getting her master’s in Systems Biology and Landscape Genetics from the University of Idaho. Jess started working as a laboratory technician with the Genome Analyzer and has since built the lab to its current configuration. Jess worked extensively on all the instrumentation that has been at the DSIL and, therefore, has a breadth of knowledge on all the sequencing platforms. Currently, she is the Research Lab Manager and oversees all work in the core.
MariaLynn Crosby
Research Specialist
MariaLynn Crosby joined the DSIL in 2017 after receiving her bachelor’s degrees in Chemistry and in Biology: Biomedical Science. ML started as a research technician with the PacBio Sequel and assisted with all the instrumentation the lab had seen. ML has delved into library design and some realms of computer analysis relating to the instruments at the DSIL.
She is currently a Research Specialist for the lab and enjoys working with researchers to design and optimize their projects.
Current Fleet
| AVITI |
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 The AVITI system is a benchtop sequencer that delivers high-quality sequencing data, with high accuracy, focusing on flexibility and affordability. It uses an innovative Avidity Sequencing technology to optimize nucleotide incorporation and identification, offering a range of sequencing kits and applications. |
| BioAnalazyer |
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 The Agilent BioAnalyzer 2100 is an automated capillary electrophoresis system used to evaluate the quality of a sample containing DNA, RNA, or proteins. It uses a microfluidics system, which reduces the amount of sample required for analysis as compared to traditional electrophoresis. |
| MiSeq |
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 The MiSeq System by Illumina is a benchtop sequencer that provides rapid and cost-effective DNA sequencing. It’s designed for a wide range of sequencing applications and can be used for tasks like amplicon sequencing, 16S metagenomics, and targeted gene sequencing. |
| MiSeq i100 |
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 The Illumina iSeq 100 is a benchtop sequencer that can generate 1.2 GB of data in only 17.5 hours. This machine is ideal for small NGS projects, such as small genome sequencing, targeted sequencing, or small panels. |
| MiniSeq |
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 The Illumina MiniSeq System is a next-generation sequencing (NGS) platform designed for cost-effective and accessible DNA and RNA sequencing. It can generate up to 7.5 GB of data per run and is known for its high-quality data output. |